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Identification of Antigens in Immune Complexes

Nozomi Aibara1, Kaname Ohyama2

  • 1Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan.

Methods in Molecular Biology (Clifton, N.J.)
|August 1, 2019
PubMed

Comprehensive identification and profiling of antigens in immune complexes (IC-antigens) is useful to provide insights into pathophysiology and could form the basis for novel diagnostic and treatment strategies for many immune-related diseases. Immune complexome analysis is the method for comprehensively identifying and profiling IC-antigens in biological fluids (such as serum and cerebrospinal fluid). Here, we describe an IC-antigen detection method; specifically, ICs in biological fluids are captured by using protein G- or protein A-coated beads, are subjected to papain-digestion, elution, and tryptic digestion, and are analyzed by nano-liquid chromatography-tandem mass spectrometry (nano-LC-MS/MS).

Keywords:
AntibodyAntigenImmune complexNano-LC-MS/MSPapain

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