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Long non-coding RNA MNX1-AS1 promoted osteosarcoma proliferation and invasion via inhibiting KISS1

Y-X Zhang1, H-X Cui, L Liu

  • 1Department of Emergency Surgery, Jining No. 1 People's Hospital, Jining, China. ygk0113@sina.com.

European Review for Medical and Pharmacological Sciences
|August 1, 2019
PubMed
Abstract

Insights

Long non-coding RNA MNX1-AS1 promotes osteosarcoma development by enhancing cell proliferation and invasion. Inhibiting MNX1-AS1 upregulates KISS1, offering a potential therapeutic target for osteosarcoma.

Area of Science:

  • Molecular biology
  • Oncology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) are implicated in human disease progression.
  • The role of lncRNA MNX1-AS1 in osteosarcoma development requires further investigation.

Purpose of the Study:

  • To elucidate the association between lncRNA MNX1-AS1 and osteosarcoma.
  • To explore the functional role and underlying mechanism of MNX1-AS1 in osteosarcoma.

Main Methods:

  • MNX1-AS1 expression was quantified in osteosarcoma tissues and cells using RT-qPCR.
  • Functional assays (proliferation, colony formation, Transwell) were performed after MNX1-AS1 knockdown.
  • Mechanism investigation involved RT-qPCR and Western blot to assess KISS1 expression.

Main Results:

  • MNX1-AS1 was significantly upregulated in osteosarcoma tissues compared to adjacent tissues.
  • Knockdown of MNX1-AS1 suppressed osteosarcoma cell proliferation and invasion in vitro.
  • Silencing MNX1-AS1 led to the upregulation of KISS1 at both mRNA and protein levels.

Conclusions:

  • MNX1-AS1 promotes osteosarcoma cell proliferation and invasion.
  • MNX1-AS1 exerts its effects by inhibiting KISS1.
  • Targeting MNX1-AS1 may represent a potential therapeutic strategy for osteosarcoma.

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