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Blueberry extract attenuates doxorubicin-induced damage in H9c2 cardiac cells 1

Yue Sun1, Ashley S Nemec-Bakk2, Azim U Mallik1

  • 1Department of Biology, Northern Ontario School of Medicine, Lakehead University, Thunder Bay, ON P7B 5E1, Canada.

Canadian Journal of Physiology and Pharmacology
|August 1, 2019
PubMed

Insights

Wild blueberries offer significant cardioprotective effects against doxorubicin-induced damage by reducing oxidative stress and apoptosis. Commercial blueberries showed minimal impact, highlighting the superior benefits of wild varieties for heart cell protection.

Area of Science:

  • Cardiovascular research
  • Pharmacology
  • Nutraceutical science

Background:

  • Doxorubicin is a potent chemotherapy agent with known cardiotoxic side effects.
  • Oxidative stress and apoptosis are key mechanisms underlying doxorubicin-induced cardiotoxicity.
  • Wild blueberries are rich in bioactive compounds with potential antioxidant and anti-inflammatory properties.

Purpose of the Study:

  • To investigate the cardioprotective potential of wild blueberry genotypes against doxorubicin-induced cellular damage.
  • To compare the efficacy of wild blueberry extracts with a commercial blueberry extract.
  • To evaluate the impact of blueberry pre-treatment on oxidative stress and apoptosis markers in cardiac cells.

Main Methods:

  • H9c2 cardiac cells were exposed to doxorubicin following pre-treatment with gallic acid equivalent blueberry extracts.
  • Analysis of oxidative stress markers and active caspase 3/7 (apoptosis marker) was performed.
  • Quantification of total phenolic content and surface area of blueberry genotypes.

Main Results:

  • Wild blueberry genotypes exhibited significantly higher total phenolic content compared to commercial blueberries.
  • Pre-treatment with wild blueberry extracts attenuated doxorubicin-induced increases in oxidative stress.
  • Wild blueberry extracts effectively reduced doxorubicin-induced caspase 3/7 activity, indicating apoptosis inhibition.

Conclusions:

  • Wild blueberry extracts demonstrate significant cardioprotective effects against doxorubicin-induced cardiotoxicity in vitro.
  • The cardioprotective action is attributed to the reduction of oxidative stress and apoptosis.
  • Commercial blueberry varieties showed limited efficacy in protecting cardiac cells from doxorubicin damage.
Keywords:
H9c2 cardiac cellsantioxidantantioxydantapoptoseapoptosisblueberriescellules cardiaques H9c2doxorubicindoxorubicinemyrtillesoxidative stressphenolsphénolsstress oxydatif

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