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Gene expression profiling reveals upregulated FUT1 and MYBPC1 in children with pancreaticobiliary maljunction

Wan-Liang Guo1, Jia Geng2, Jun-Gang Zhao3

  • 1Department of Radiology, Children's Hospital of Soochow University, Suzhou, China.

Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Medicas E Biologicas
|August 1, 2019
PubMed

Insights

Pancreaticobiliary maljunction (PBM) in children shows altered gene expression. FUT1 and MYBPC1 are upregulated and may serve as predictive biomarkers for PBM.

Area of Science:

  • Pediatric Gastroenterology
  • Molecular Biology
  • Genetics

Background:

  • Pancreaticobiliary maljunction (PBM) increases the risk of bile duct and gallbladder cancer.
  • Genetic expression changes in children with PBM have not been thoroughly studied.

Purpose of the Study:

  • To investigate genome-wide gene expression differences in children with PBM.
  • To identify potential biomarkers for early detection of PBM.

Main Methods:

  • Genome-wide expression analysis using peripheral blood from 10 children with PBM and 15 controls.
  • Identification of differentially expressed genes (DEGs) via microarray.
  • Bioinformatics analysis (Gene Ontology, KEGG) and qRT-PCR validation.
  • Receiver operating characteristic (ROC) curve analysis for biomarker accuracy.

Main Results:

  • Identified 876 DEGs in PBM patients (530 upregulated, 346 downregulated).
  • Confirmed upregulation of MYBPC1 and FUT1 in PBM.
  • ROC analysis indicated high predictive accuracy for FUT1 (AUC=0.873) and MYBPC1 (AUC=0.960).

Conclusions:

  • FUT1 and MYBPC1 are significantly upregulated in children with PBM.
  • Upregulated FUT1 and MYBPC1 show potential as predictive biomarkers for PBM in pediatric patients.

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