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Magnetized Carbon Nanotube Based Lateral Flow Immunoassay for Visual Detection of Complement Factor B
Yan Huang1,2,3, Tingting Wu1, Fang Wang1
1Research Center for Bioengineering and Sensing Technology, University of Science & Technology Beijing, Beijing 100083, China.
Molecules (Basel, Switzerland)
|August 2, 2019
Summary
A new magnetized carbon nanotube (MCNT) lateral flow immunoassay (LFI) enables visual detection of complement factor B (CFB) in blood. This rapid, low-cost MCNT-LFI tool offers direct detection in human blood.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Immunosensors
Background:
- Complement factor B (CFB) plays a crucial role in the complement system.
- Accurate and rapid detection of CFB is important for diagnosing various diseases.
- Existing detection methods can be time-consuming and expensive.
Purpose of the Study:
- To develop a novel magnetized carbon nanotube (MCNT) based lateral flow immunoassay (LFI).
- To enable rapid, visual, and cost-effective detection of complement factor B (CFB) in human blood.
- To establish a proof-of-concept for MCNT-LFI in direct blood sample analysis.
Main Methods:
- Preparation of MCNT by decorating Fe3O4 nanoparticles on multi-walled CNTs.
- Covalent immobilization of anti-CFB monoclonal antibody (mAb) onto MCNT surface.
- Development of a lateral flow strip using mAb-functionalized MCNT and anti-CFB polyclonal antibody.
- Assay procedure involving magnetic separation and visual band detection with a portable reader.
Main Results:
- The MCNT-based LFI successfully detected CFB in buffer with a detection limit of 5 ng mL-1.
- CFB in 1 μL of human blood was detected within 30 minutes.
- The assay results showed high correlation with commercial ELISA kits.
- Visual detection of characteristic brown bands was achieved.
Conclusions:
- The MCNT-based LFI is a rapid and sensitive tool for CFB detection in whole blood.
- This method offers a low-cost alternative to existing diagnostic techniques.
- The MCNT-LFI has potential for point-of-care applications in clinical diagnostics.
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