Functional consequences of enhanced expression of STIM1 and Orai1 in Huh-7 hepatocellular carcinoma tumor-initiating cells

The endoplasmic reticulum (ER) Ca²⁺ sensor, stromal interaction molecule1 (STIM1) activates the plasma membrane (PM) channel Orai1 in order to mediate store-operated Ca²⁺ entry (SOCE) in response to ER store depletion. Enhanced expression of STIM1 in cancer tissue has been associated with poor patient prognosis. Therefore, this study investigated the functional consequences of enhanced expression of STIM1 and Orai1 in a tumor-initiating subpopulation of Huh-7 hepatocellular carcinoma (HCC) cells that express epithelial cell adhesion molecule (EpCAM) and Prominin 1 (CD133).

We performed qRT-PCR, intracellular Ca²⁺ monitoring, protein analyses, and real-time cell proliferation assays on EpCAM(+)CD133(+) subpopulation of tumor-initiating Huh-7 HCC cells expressing high levels of STIM1 and/or Orai1. Statistical significance between the means of two groups was evaluated using unpaired Student's t-test.

Enhanced STIM1 expression significantly increased ER Ca²⁺ release and proliferation rate of EpCAM(+)CD133(+) cells.

STIM1 overexpression may facilitate cancer cell survival by increasing ER Ca²⁺-buffering capacity, which makes more Ca²⁺ available for the cytosolic events, on the other hand, possibly preventing Ca²⁺-dependent enzymatic activity in mitochondria whose Ca²⁺ uniporter requires much higher cytosolic Ca²⁺ levels.