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Related Experiment Videos

Ascorbic acid status: biochemical and clinical considerations.

W Lee1, K A Davis, R L Rettmer

  • 1Department of Laboratory Medicine, University of Washington, Seattle 98195.

The American Journal of Clinical Nutrition
|August 1, 1988
PubMed
Summary
This summary is machine-generated.

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Ascorbic acid (AA) and its oxidized form (DHAA) stability varies by condition. Factors like temperature and glucose impact AA status indicators, suggesting a need for better assessment methods.

Area of Science:

  • Biochemistry
  • Nutritional Science
  • Clinical Chemistry

Background:

  • Ascorbic acid (AA) is a vital nutrient with antioxidant properties.
  • Dehydroascorbic acid (DHAA) is the oxidized form of AA.
  • Understanding AA and DHAA stability is crucial for accurate assessment.

Purpose of the Study:

  • To investigate the stability of Ascorbic acid (AA) and dehydroascorbic acid (DHAA) under various conditions.
  • To evaluate factors affecting AA and DHAA levels in biological samples and solutions.
  • To assess the reliability of current indicators for vitamin C status.

Main Methods:

  • Assessed AA + DHAA stability in whole blood and plasma at different temperatures.
  • Determined the half-life of AA + DHAA in total parenteral nutrition solutions.

Related Experiment Videos

  • Developed a dithiothreitol reduction method for quantifying total AA and DHAA.
  • Measured AA content in polymorphonuclear leukocytes (PMNs) under various physiological stresses.

    • Main Results:

    • AA + DHAA was stable in unopened vacutainer tubes.
    • DHAA oxidation in plasma was rapid and irreversible at 25°C but stable at 4°C.
    • The half-life of AA + DHAA in parenteral nutrition solutions exceeded 24 hours.
    • DHAA constituted less than 5% of total plasma AA in healthy and diabetic individuals.
    • Cellular AA content per PMN decreased under stress, despite no change per unit of blood.
    • Increased glucose concentration doubled the Km for AA uptake by PMNs.

    Conclusions:

    • Ascorbic acid and dehydroascorbic acid stability is condition-dependent.
    • Temperature significantly impacts DHAA stability in plasma.
    • Factors like stress and glucose levels influence vitamin C uptake and cellular content.
    • Current methods may not provide a reliable indicator of overall ascorbic acid status.