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Development of Compendium for Esophageal Squamous Cell Carcinoma
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P53/MDM2 Co-Expression in Laryngeal Squamous Cell Carcinoma Based on Digital Image Analysis
Aristeidis Chrysovergis1, Vasileios Papanikolaou1, Evangelos Tsiambas2
1First Department of Otorhinolaryngology, Head and Neck Surgery, Hippocration Hospital, University of Athens, Athens, Greece.
Anticancer Research
|August 2, 2019
Summary
Overexpression of P53 and murine double minute 2 (MDM2) proteins correlates with advanced laryngeal squamous cell carcinoma (LSCC) stages. MDM2 overexpression is frequent in LSCC and linked to poorer patient survival.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- P53 acts as a tumor suppressor, regulating genomic stability.
- Murine double minute 2 (MDM2) is a proto-oncogene interacting with P53 in an auto-regulatory pathway.
- Laryngeal squamous cell carcinoma (LSCC) is a significant disease requiring better understanding of its molecular drivers.
Purpose of the Study:
- To investigate the correlation between P53 and MDM2 expression in LSCC.
- To assess the relationship between P53/MDM2 expression and clinicopathological features of LSCC.
- To evaluate the prognostic significance of P53 and MDM2 in LSCC patients.
Main Methods:
- Immunohistochemistry was used to detect P53 and MDM2 protein expression in 50 LSCC tissue samples.
- Digital image analysis quantified protein expression levels, including staining intensity and densitometry.
- Statistical analysis correlated protein expression with LSCC stage and patient survival.
Main Results:
- P53 overexpression was found in 32% of LSCC cases, and MDM2 overexpression in 44%.
- Combined P53/MDM2 overexpression occurred in 26% of cases.
- Strong positive correlation between P53 and MDM2 expression (p=0.001). Both were linked to advanced LSCC stage (p=0.032 and p=0.001). MDM2 overexpression correlated with poorer survival (p=0.046).
Conclusions:
- Aberrant co-expression of P53 and MDM2 is associated with advanced LSCC.
- MDM2 overexpression is a frequent event in LSCC and negatively impacts patient survival.
- Targeting the P53-MDM2 pathway may offer therapeutic strategies for LSCC.
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