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Updated: Jan 21, 2026

Live Imaging of GFP-labeled Proteins in Drosophila Oocytes
Published on: March 29, 2013
Data on Drosophila clots and hemocyte morphologies using GFP-tagged secretory proteins: Prophenoloxidase and
Alexis Dziedziech1, Martin Schmid1, Badrul Arefin1
1Stockholm University, Department of Molecular Biosciences, Svante Arrhenius Väg 20C, SE, 10691 Sweden.
Abstract:
Insect hemolymph coagulation: Kinetics of classically and non-classically secreted clotting factors Schmid et al., 2019. The linked article demonstrates the localization of two secretory proteins in Drosophila melanogaster, Prophenoloxidase (PPO2) and Transglutaminase-A (Tg) in hemocytes as well the clot with different tissue-specific drivers. Here we provide further data for the usefulness of the GFP-tagged version of the two crosslinking enzymes that are involved in clot hardening. The morphology of crystal cells is described using GFP-tagged PPO2 rather than with the use of antibodies in ex vivo hemolymph preparations. The use of the GFP-tagged proteins PPO2 and Tg is shown in additional contexts.
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