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Related Experiment Video

Updated: Jan 21, 2026

Bioenergetic Profile Experiment using C2C12 Myoblast Cells
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Electrostatically localized proton bioenergetics: better understanding membrane potential.

James Weifu Lee1

  • 1Department of Chemistry and Biochemistry, Old Dominion University, Norfolk, VA 23529 USA.

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|August 2, 2019
PubMed
Summary
This summary is machine-generated.

The origin of membrane potential is clarified as electrostatic surface charge density, primarily from localized protons. This understanding refines Mitchell

Keywords:
ATP synthesisBiochemistryBioenergeticsBiophysicsBiotechnologyCell biologyComputational biologyElectrostatically localized protonsLiquid-membrane interfaceLocalized surface charge densityMembrane potentialMicrobiologyMitochondriaProtonic capacitorStructural biology

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Area of Science:

  • Biophysics
  • Electrochemistry
  • Cellular Biology

Background:

  • Mitchell's chemiosmotic theory described membrane potential as a transmembrane electric potential difference.
  • The precise physical origin of membrane potential within this theory remained incompletely explained.

Purpose of the Study:

  • To elucidate the physical origin of membrane potential using the Lee proton electrostatic localization model.
  • To reformulate the protonic motive force (pmf) equation incorporating electrostatically localized protons.

Main Methods:

  • Application of the Lee proton electrostatic localization model.
  • Formulation of a new equation for protonic motive force (pmf).
  • Analysis of localized surface charge density at the liquid-membrane interface.

Main Results:

  • Membrane potential is understood as the voltage from localized surface charge density at the liquid-membrane interface, akin to a capacitor.
  • This charge density comprises electrostatically localized protons and non-proton cations.
  • Localized protons constitute approximately 78% of the total localized surface charge density in animal mitochondria at equilibrium.

Conclusions:

  • The physical origin of membrane potential is the electrostatic formation of localized surface charge density.
  • Cation-proton exchange processes do not alter the total localized charge density or membrane potential.
  • Liquid water's role as a protonic conductor in membrane potential formation and utilization is significant.