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Updated: Jan 21, 2026

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
Published on: November 21, 2010
Disulfide-Containing Detergents (DCDs) for the Structural Biology of Membrane Proteins
Dongxiang Xue1,2,3,4, Tiandan Xu1,3,4,5, Huixia Wang1,3,4,5
1iHuman Institute, ShanghaiTech University, Ren Building, 393 Middle Huaxia Road, Pudong New District, Shanghai, 201210, China.
Abstract:
Disulfide-containing detergents (DCDs) are introduced, which contain a disulfide bond in the hydrophobic tail. DCDs form smaller micelles than corresponding detergents with linear hydrocarbon chains, while providing good solubilization and reconstitution of membrane proteins. The use of this new class of detergents in structural biology is illustrated with solution NMR spectra of the human G protein-coupled receptor A2A AR, which is an α-helical protein, and the β-barrel protein OmpX from E. coli.
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