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Updated: Jan 21, 2026

Mesocosm-Scale Constructed Wetland Design for Wastewater Treatment
Published on: May 2, 2025
Removal of the endocrine disruptors ethinyl estradiol, bisphenol A, and levonorgestrel by subsurface constructed
Julyenne Meneghetti Campos1, Sonia C N Queiroz2, Denis M Roston1
1School of Agricultural Engineering, University of Campinas, Candido Rondon Avenue, 501, 13083-875 Campinas, SP, Brazil.
Abstract:
The present work aimed to evaluate the removal efficiency of the endocrine disruptors ethinyl estradiol (EE2), the progestin levonorgestrel (LNG), and bisphenol A (BPA), considered to be contaminants of major concern, by using four laboratory scale constructed wetlands (CW) - three containing gravel as support media, one cultivated with Cyperus isocladus (WL1), other with Eichhornia crassipes (WL3), and one without macrophyte (WL2). The fourth unit contained gravel and bamboo charcoal as support medium, also cultivated with Cyperus isocladus (WLC). Two hydraulic retention times (HRT) were tested, 2 and 4 days. Average removals ranged from 9.0 to 95.6% for EE2, from 29.5 to 91.2% for BPA and from 39.1 to 100.0% for LNG. The results showed that the most efficient CW for removal of EE2 and BPA was WLC, and for LNG removal was WL3. The 2 days HRT was statistically more efficient in removing EE2, and the 4 days HRT was more efficient in the LNG removal. The other endocrine disruptors and concentration ranges were not influenced by HRT. It was concluded that WLC was the most suitable CW for removal of these three compounds, and it possibly is efficient also for the removal of other endocrine disruptors with similar physical-chemical characteristics.
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