Toxicity response of highly colloidal, bioactive, monodisperse SiO₂@ Pr(OH)₃ hollow microspheres

In a facile synthesis, highly colloidal, bioactive Pr(OH)₃-encapsulated silica microspheres (PSMSs) with an average diameter of 500-700 nm were successfully prepared via a sol-gel process followed by heat treatment. The phase formation, morphology, surface and optical properties of the as-synthesized PSMSs were characterized by various techniques including X-ray diffraction (XRD), thermogravimetric analysis (TGA), scanning electron microscope (SEM), transmission electron microscope (TEM), N₂-adsorption-desorption, energy dispersive X-ray (EDX) analysis, Fourier transform infrared (FTIR) and UV/vis spectroscopy. The PSMSs were semi-amorphous or ultra-small in size, highly dispersible in water, mesoporous, irregular in size and spherical in shape. The SEM images show a well-ordered broad nanoporous structure which is preserved after coating with Pr(OH)₃ molecules, demonstrating interaction between the optically active Pr³⁺ ion and silanol (Si-OH) groups via hydrogen bonding. Optical spectra show well-resolved weak intensity 4f-4f absorption transitions in the visible region of the Pr³⁺ ion, indicating successful grafting of the Pr(OH)₃ layer. Toxicity was measured by MTT and NRU assays to determine potential toxicity. Cell viability was suppressed with increasing dosage of PSMSs, but showed greater than 55% cell viability at a concentration of 200 μg/mL, resulting in low toxicity. Due to its high aqueous dispersibility, optical activity, excellent biocompatibility and low toxic nature, it could be a favorable material for biomedical and drug delivery applications.