Related Experiment Video
Updated: Jan 21, 2026

Preparation of Mitochondria from Ovarian Cancer Tissues and Control Ovarian Tissues for Quantitative Proteomics Analysis
Published on: November 18, 2019
Sequence variants in FSHR and CYP19A1 genes and the ovarian response to controlled ovarian stimulation
Di Song1, Xian-Liang Huang2, Ling Hong3
1NHC Key Laboratory of Reproduction Regulation, Shanghai Institute of Planned Parenthood Research, Medical School, Fudan University, Shanghai, People's Republic of China; Second Military Medical University, Changhai Hospital, Shanghai, People's Republic of China.
Objective:
To examine whether sequence variants within the FSHR and CYP19A1 genes are related to the ovarian response to controlled ovarian stimulation (COS).
Design:
Genetic association study using both single-gene and combined analyses of women with sequence variants undergoing in vitro fertilization treatment.
Setting:
Academic research institute hospital.
Patient(S):
Seven hundred and five women undergoing ovarian stimulation with recombinant follicle-stimulating hormone (FSH).
Intervention(S):
Peripheral blood extraction, DNA purification, and FSHR c.919G>A (rs6165, p.Thr307Ala) and CYP19A1 c.*19C>T (rs10046) sequence variants analyses.
Main Outcome Measure(S):
Single-gene statistical analysis and combined statistical analysis with the SPSS17.0 software; FSHR c.919G>A and CYP19A1 c.*19C>T sequence variant genotypes and clinical parameters related to the COS response as oocyte retrieval and hormone levels, doses of exogenous FSH.
Result(S):
Women with genotype Ala/Ala at FSHR position 307 had higher basal levels of FSH and were more likely to have a low ovarian response compared with other genotypes. Women with genotype TT at CYP19A1 yielded fewer oocytes after ovarian stimulation. The combined analysis of these two sequence variants revealed that these two single-nucleotide variants have a synergistic effect in conferring the risk of a low ovarian response.
Conclusion(S):
Our results support an association of sequence variants in the genes that participate in estrogen synthesis, notably the FSHR and CYP19A1 genes, with the outcome of COS.
More Related Videos
Related Concept Videos
Hormonal Control of the Ovarian Cycle
Before puberty, the hypothalamus releases GnRH in a low frequency, low amplitude pulsatile manner. This along with the immature hypothalamic-pituitary-gonadal axis activity, results in low estrogen levels and the absence of a fully functional ovarian cycle. At puberty, GnRH secretion increases in both frequency and...
Ovarian Cycle
Combinatorial Gene Control
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
Histone Variants at the Centromere
Gene Flow
Cis-regulatory Sequences

