Related Experiment Video
Updated: Jan 21, 2026
10:36
Systems Biology of Metabolic Regulation by Estrogen Receptor Signaling in Breast Cancer
Published on: March 17, 2016
10.9K
Serotonin receptor oligomerization regulates cAMP-based signaling
Sonal Prasad1, Evgeni Ponimaskin1,2, Andre Zeug3
1Cellular Neurophysiology, Center of Physiology, Hannover Medical School, Carl-Neuberg-Straße 1, 30625 Hannover, Germany.
Journal of Cell Science
|August 3, 2019
Summary
Serotonin receptors 5-HT7R and 5-HT1AR form complexes that alter cAMP signaling. Receptor ratios in neurons may influence psychiatric disorders like anxiety and depression.
Area of Science:
- Neuroscience
- Molecular Biology
- Pharmacology
Background:
- Protein-protein interactions are crucial for cellular signaling.
- Förster resonant energy transfer (FRET) is a key technique for studying molecular interactions.
- Serotonin receptors play vital roles in neurological functions and psychiatric disorders.
Purpose of the Study:
- To investigate the oligomerization of 5-HT7 receptor (5-HT7R) and 5-HT1A receptor (5-HT1AR).
- To determine the impact of this hetero-oligomerization on cAMP signaling.
- To understand the role of receptor stoichiometry in endogenous serotonergic signaling.
Main Methods:
- Combined linear unmixing FRET (lux-FRET) with a FRET-based biosensor for cAMP.
- Quantitative molecular microscopy.
- Kinetic modeling of intracellular cAMP level changes.
Main Results:
- 5-HT7R stimulates cAMP production and forms hetero-oligomers with 5-HT1AR.
- Hetero-oligomerization blocks the inhibitory effect of 5-HT1AR on cAMP signaling.
- 5-HT7R signaling is unaffected by hetero-oligomerization.
Conclusions:
- Serotonergic signaling is modulated by individual receptor concentration and interactions with other receptors.
- The ratio of serotonin receptors is critical for neuronal function and may influence psychiatric conditions.
- Findings provide insights into the complex signaling of serotonin receptors in health and disease.
Keywords:
5-HT1AR5-HT7REpacFRETFörster resonance energy transferG protein-coupled receptorGPCRHetero-oligomerizationLux-FRETQuantitative confocal microscopyStoichiometrycAMP signalingMore Related Videos
Related Concept Videos
01:23Drugs Affecting GI Tract Motility: Serotonin Receptor Agonists
912
Serotonin, a crucial neurotransmitter synthesized by enterochromaffin cells, plays a cardinal role in regulating gastrointestinal (GI) motility. With over 90% of the body's total serotonin in the GI tract, its influence on digestive processes is profound. Serotonin is swiftly released upon various stimuli, such as food boluses or certain drugs, triggering intrinsic sensory neurons in the myenteric plexus and extrinsic vagal and spinal sensory neurons. This leads to the activation of the...
912
01:41Signal Sequences and Sorting Receptors
14.9K
Signal sequences are short amino acid sequences that guide newly synthesized proteins to their proper location within the cell. Classical signal sequences are fifteen to sixty amino acids long and present at the N-terminus of a polypeptide chain. Each signal sequence has a conserved segment of basic residues towards their N terminus, a hydrophobic core, and a C-terminus rich in polar residues. The C-terminus also contains a signal cleavage site and features a -3 -1 sequence motif. The -3-1...
14.9K
01:28Insulin: The Receptor and Signaling Pathways
2.8K
Insulin action is mediated through a receptor tyrosine kinase, akin to the IGF-1 receptor. The number of receptors per cell varies significantly, from 40 on erythrocytes to 300,000 on adipocytes and hepatocytes. The insulin receptor consists of linked α/β subunit dimers, forming a heterotetramer glycoprotein with two extracellular α subunits and two β subunits spanning the membrane. The α subunits inhibit the inherent tyrosine kinase activity of the β subunits, but...
2.8K
02:14GTPases and their Regulation
9.8K
Guanine nucleotide-binding proteins (G-proteins), also known as GTPases, are a superfamily of proteins that regulate many cellular processes, such as cell signaling, vesicular transport, and the regulation of cell shape and motility. Mutation or dysfunction of these proteins can lead to disease. There are around 40,000 known G-proteins that can broadly be classified into two groups ‒ small G-proteins consisting of a single domain and large multi-domain G-proteins.
Large G-proteins,...
Large G-proteins,...
9.8K
01:24Intracellular Signaling Cascades
53.3K
Once a ligand binds to a receptor, the signal is transmitted through the membrane and into the cytoplasm. The continuation of a signal in this manner is called signal transduction. Signal transduction only occurs with cell-surface receptors, which cannot interact with most components of the cell, such as DNA. Only internal receptors can interact directly with DNA in the nucleus to initiate protein synthesis. When a ligand binds to its receptor, conformational changes occur that affect the...
53.3K
02:03What is Cell Signaling?
129.9K
Despite the protective membrane that separates a cell from the environment, cells need the ability to detect and respond to environmental changes. Additionally, cells often need to communicate with one another. Unicellular and multicellular organisms use a variety of cell signaling mechanisms to communicate to respond to the environment.
129.9K