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Updated: Jan 21, 2026

Microscopy of Fission Yeast Sexual Lifecycle
Published on: March 9, 2016
Subunit interactions and arrangements in the fission yeast Mis16-Mis18-Mis19 complex
Melanie Korntner-Vetter1, Stéphane Lefèvre1, Xiao-Wen Hu1
1Structural Biology of Chromosome Segregation Laboratory, The Francis Crick Institute, London, UK.
Abstract:
Centromeric chromatin in fission yeast is distinguished by the presence of nucleosomes containing the histone H3 variant Cnp1CENP-A Cell cycle-specific deposition of Cnp1 requires the Mis16-Mis18-Mis19 complex, which is thought to direct recruitment of Scm3-chaperoned Cnp1/histone H4 dimers to DNA. Here, we present the structure of the essential Mis18 partner protein Mis19 and describe its interaction with Mis16, revealing a bipartite-binding site. We provide data on the stoichiometry and overall architecture of the complex and provide detailed insights into the Mis18-Mis19 interface.
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