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Evaluation of Intracellular Location of Reactive Oxygen Species in Solea Senegalensis Spermatozoa
Published on: March 11, 2018
The roles of reactive oxygen species and antioxidants in cryopreservation
Jia Soon Len1, Wen Shuo Darius Koh1, Shi-Xiong Tan2
1Diploma in Biomedical Science, School of Applied Science, Republic Polytechnic, 9 Woodlands Avenue 9, Singapore 738964.
Abstract:
Cryopreservation has facilitated advancement of biological research by allowing the storage of cells over prolonged periods of time. While cryopreservation at extremely low temperatures would render cells metabolically inactive, cells suffer insults during the freezing and thawing process. Among such insults, the generation of supra-physiological levels of reactive oxygen species (ROS) could impair cellular functions and survival. Antioxidants are potential additives that were reported to partially or completely reverse freeze-thaw stress-associated impairments. This review aims to discuss the potential sources of cryopreservation-induced ROS and the effectiveness of antioxidant administration when used individually or in combination.
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