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The increasing threat of silver-resistance in clinical isolates from wounds and burns

Alaa El-Dien Ms Hosny1, Salwa A Rasmy1, Dina S Aboul-Magd2

  • 1Microbiology and Immunology Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt.

Infection and Drug Resistance
|August 3, 2019
PubMed
Abstract

Insights

Silver resistance in bacteria is increasing due to widespread silver compound use. This study detected plasmid-mediated silver resistance in clinical isolates, highlighting the need for monitoring silver use in healthcare.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Widespread use of silver compounds has led to the emergence of silver-resistant bacteria.
  • Limited data exists on plasmid-mediated silver resistance in developing countries.

Purpose of the Study:

  • To detect silver resistance in wound and burn isolates.
  • To genetically characterize plasmid-mediated silver resistance genes (sil genes).

Main Methods:

  • Bacterial isolates were identified and their antimicrobial susceptibility tested.
  • Minimum inhibitory concentration (MIC) for silver nitrate (AgNO3) was determined.
  • Polymerase chain reaction (PCR) and plasmid transformation were used to investigate sil genes and resistance transfer.

Main Results:

  • Nineteen (12.6%) silver-resistant isolates were detected, including Klebsiella pneumoniae, Staphylococcus aureus, Escherichia coli, Enterobacter cloacae, Pseudomonas aeruginosa, and Acinetobacter baumannii.
  • Different sil genes were found on plasmids, and plasmid transformation successfully transferred silver resistance.
  • Extracted plasmids exhibited diverse replicon types.

Conclusions:

  • Plasmid-mediated silver resistance was identified in P. aeruginosa, A. baumannii, and S. aureus for the first time, alongside K. pneumoniae, E. coli, and Enterobacter cloacae.
  • Strict monitoring of silver compound use in medical settings is recommended.
  • Implementation of standardized silver resistance detection methods is necessary.
Keywords:
multidrug-resistanceplasmid-mediatedreplicon typesil genes

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