Related Experiment Video
Updated: Jan 21, 2026

Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth
Published on: May 17, 2024
Isolation and culture of dental pulp stem cells from permanent and deciduous teeth
Shagufta Naz1, Farhan Raza Khan2, Raheela Rahmat Zohra3
1Ms. Shagufta Naz, M.Sc., Department of Surgery, Department of Biotechnology, University of Karachi, Pakistan. Aga Khan University, Karachi, Pakistan.
Objective:
To isolate dental pulp mesenchymal stem cells (MSCs) from non-infected human permanent and deciduous teeth.
Methods:
It was an in-vitro experimental study. Human teeth were collected from 13 apparently healthy subjects including nine adults and four children. After decoronation dental pulps were extirpated from teeth and cultured via explant method in a stem cell defined media. Data was analyzed by descriptive statistics.
Results:
As above MSCs emerged exhibiting fibroblast-like morphology. In vitro culture was positive for 100% (9/9) and 75% (3/4) of the permanent and deciduous teeth respectively. First cell appeared from deciduous teeth pulp in 10±6.2 days while permanent teeth pulp took 12.4±3.7 days. Together, 26.6±3.6 and 24.5±3.5 days were required for permanent and deciduous tooth pulp stem cells to be ready for further assays.
Conclusions:
The protocol we developed is easy and consistent and can be used to generate reliable source of MScs for engineering of calcified and non-calcified tissue for regenerative medicine approaches.
More Related Videos
14:52Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
Published on: November 24, 2012
03:45Author Spotlight: Advancing Tissue Regeneration and Disease Modeling with Dental Pulp Stem Cells
Published on: May 5, 2023
Related Concept Videos
Teeth
In the bud stage, the tooth germ (an aggregation of cells) starts to form in the developing jawbone. During the cap stage, the tooth germ differentiates into enamel organ, dental papilla, and dental sac, which will later develop into the tooth's enamel, dentin...
Stem Cell Culture
Embryonic Stem Cells
Embryonic Stem Cells
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
Induced Pluripotent Stem Cells
Adult Stem Cells