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Updated: Jan 21, 2026

Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages
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Data on autophagy markers in human macrophages exposed to oxLDL and growth differentiation factor-15

Kathrin Ackermann1, Gabriel A Bonaterra1, Ralf Kinscherf1

  • 1Institute for Anatomy and Cell Biology, Department of Medical Cell Biology, Philipps-University of Marburg, 35032 Marburg, Germany.

Data in Brief
|August 3, 2019
PubMed

Insights

Growth differentiation factor-15 (GDF-15) influences autophagy in human macrophages during foam cell formation. This cytokine impacts lipid homeostasis and may play a role in atherosclerosis progression.

Area of Science:

  • Molecular Biology
  • Immunology
  • Cardiovascular Research

Background:

  • Growth differentiation factor-15 (GDF-15), also known as macrophage-inhibitory cytokine-1 (MIC-1), is linked to cardiovascular disease, inflammation, and atherosclerosis.
  • Macrophages (MΦ) in atherosclerotic lesions highly express GDF-15.
  • Macrophage uptake of oxidized-low density lipoprotein (oxLDL) and autophagic processes are implicated in arteriosclerotic progression.

Purpose of the Study:

  • To investigate the regulatory effect of GDF-15 on the autophagy signaling pathway in human macrophages.
  • To explore GDF-15's role in foam cell formation and its relation to oxLDL uptake and autophagy.

Main Methods:

  • Western blot analysis was employed to assess key autophagy markers.
  • Levels of ATG5, ATG12/ATG5-complex, and p62 were measured in relation to GDF-15 concentration.

Main Results:

  • Western blot data demonstrated changes in ATG5, ATG12/ATG5-complex, and p62 expression in response to GDF-15.
  • These findings suggest GDF-15 modulates autophagy signaling in human macrophages.

Conclusions:

  • GDF-15 plays a regulatory role in the autophagy signaling pathway within human macrophages.
  • The study provides insights into GDF-15's involvement in macrophage function relevant to atherosclerosis.

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