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Updated: Jan 21, 2026

Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences
Published on: February 11, 2019
Screening dataset of food components that enhance transcriptional activity of PGC1-beta
Shiho Nakai1, Ran Uchitomi1, Rintaro Matsuda1
1Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto, Japan.
Abstract:
PGC-1β is a transcriptional co-activator of nuclear receptors, which acts to increase energy expenditure. PGC-1β fused to GAL4 DNA-binding domain transfected in HEK293T cells showed a reporter luciferase activity. We screened food-derived and natural compounds using a reporter assay system to measure the transcriptional activity of PGC-1β. We found that soy-derived isoflavones, genistein and daidzein, and several resveratrols activated PGC-1β, see "Genistein, daidzein, and resveratrols stimulate PGC-1β-mediated gene expression" [1]. The list of 166 compounds and their reporter activity is shown here.
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