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Dataset for the spore surface proteome and hydrophobin A/RodA proteoforms of A.flavus

Mohammed Razeeth Shait Mohammed1, Muthu Kumar Balamurgan1, Rabbind Singh Amrathlal2

  • 1Department of Proteomics, Aravind Medical Research Foundation, Dr. G. Venkataswamy Eye Research Institute, Aravind Eye Care System, Madurai, TamilNadu, India.

Data in Brief
|August 3, 2019
PubMed

Insights

This study reveals distinct surface protein profiles in Aspergillus flavus, a common cause of fungal keratitis. Researchers identified two unique forms of the RodA protein and the mechanism behind their generation.

Area of Science:

  • Mycology
  • Ophthalmology
  • Proteomics

Background:

  • Fungal keratitis is a significant cause of vision loss, particularly in tropical regions like India.
  • Aspergillus flavus and Fusarium are primary pathogens responsible for mycotic keratitis.

Purpose of the Study:

  • To analyze the surface proteome of Aspergillus flavus from both environmental and clinical sources.
  • To identify and characterize different proteoforms of the RodA protein in Aspergillus flavus.

Main Methods:

  • Extraction of conidial surface proteins from Aspergillus flavus isolates (saprophytic and clinical).
  • High-resolution mass spectrometry for proteomic analysis.
  • Detailed examination of RodA protein bands from polyacrylamide gels.

Main Results:

  • Significant differences were observed in the surface proteome between clinical and saprophytic Aspergillus flavus isolates, indicating ecotype-specific alterations.
  • Two distinct proteoforms of the RodA protein were identified.
  • The mechanism responsible for the generation of these two RodA proteoforms was elucidated.

Conclusions:

  • The study highlights ecotype-specific variations in the Aspergillus flavus surface proteome.
  • The identification of RodA proteoforms and their generation mechanism provides new insights into fungal biology and pathogenesis.

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