Data on Quantitative Microbial Elemental Cycling (QMEC) primer design and validation
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Updated: Jan 21, 2026

Visualizing Methane-Cycling Microbial Dynamics in Coastal Wetlands
Published on: January 31, 2025
Bang-Xiao Zheng1,2, Kai Ding1
1Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, PR China.
Abstract:
This work included the primer design details of Quantitative Microbial Elemental Cycling (QMEC) method, a high-throughput qPCR method for microbial carbon (C), nitrogen (N), phosphorus (P), sulfur (S) and methane metabolism potential detection and assessment. We designed 36 novel primers based on their amino acid sequences. Via illumina sequencing technology, their phylogenetic taxonomy was identified and analyzed to validate the primer specificity.
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