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Updated: Jan 21, 2026

Comparison of Methods for Isolating Entomopathogenic Fungi from Soil Samples
Published on: January 6, 2022
A high-throughput screening method based on the Mizoroki-Heck reaction for isolating itaconic acid-producing fungi
Mei Sano1, Hikari Kuroda1, Hitomi Ohara1
1Department of Biobased Materials Science, Kyoto Institute of Technology, 1 Hashigami-cho, Matsugasaki, Sakyo-ku, Kyoto, 606-8585, Japan.
Abstract:
In this study, we report a novel method based on the Mizoroki-Heck reaction followed by an iodine test for the screening of itaconic acid-producing fungi from soils. This method is simple, rapid, and requires 10 μL of culture; results are obtained within 1.5 h. The detection limit of itaconic acid in the cultures was 0.13 mM. This is the first report on the direct screening of itaconic acid-producing fungi using a coupling reaction.
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