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Related Experiment Videos

Intracellular interference with antigen presentation.

F Leyva-Cobian1, E R Unanue

  • 1Department of Pathology, Washington University School of Medicine, St. Louis 63110.

Journal of Immunology (Baltimore, Md. : 1950)
|September 1, 1988
PubMed
Summary
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Macrophage uptake of various molecules, including heat-killed Listeria, can block antigen presentation. This interference affects antigen processing, not MHC class II availability.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Macrophages play a crucial role in antigen presentation to T cells.
  • Intracellular processing of antigens is essential for effective T cell activation.

Purpose of the Study:

  • To investigate how macrophage uptake of certain molecules affects antigen presentation.
  • To determine the mechanism behind Listeria-induced interference with antigen presentation.

Main Methods:

  • Murine peritoneal macrophages were cultured with heat-killed Listeria monocytogenes and hen egg white lysozyme.
  • Antigen presentation assays were performed using T cells.
  • Uptake, catabolism, and surface MHC class II molecule availability were assessed.

Main Results:

  • Listeria uptake interfered with lysozyme presentation without affecting uptake or catabolism.

Related Experiment Videos

  • This interference was dependent on the order of antigen and Listeria exposure.
  • Other retained molecules also impaired lysozyme presentation, but not presentation of a processed peptide.

    • Conclusions:

    • Retention of diverse molecules within macrophages can disrupt intracellular events required for immunogenic determinant formation.
    • Interference is independent of antigen catabolism or MHC class II molecule availability.