iPSC Bioprinting: Where are We at?

Area of Science:

• Regenerative Medicine
• Biotechnology
• Stem Cell Biology

Background:

• Induced pluripotent stem cells (iPSC) offer abundant cell sources and differentiation potential.
• Bioprinting iPSC-derived cells with biomaterials creates tissue constructs for regenerative medicine.
• This technology holds promise for patient-specific treatments.

Purpose of the Study:

• To review current 3D bioprinting technologies applied to iPSC.
• To analyze the advantages, disadvantages, and parameters influencing iPSC bioprinting.
• To discuss iPSC sources, bioinks, and applications in tissue engineering.

Main Methods:

• Comprehensive literature search of major scientific databases (PubMed/MEDLINE, Google Scholar, Scopus).
• Analysis of studies on iPSC generation, undifferentiated iPSC, and bioprinting techniques.
• Review of data concerning bioprinting of iPSC for cartilage, bone, heart, neural, skin, and hepatic tissues.

Main Results:

• Bioprinting of iPSC and iPSC-derived cells enables fabrication of tissue-mimicking constructs.
• Key factors influencing cell viability, such as bioprinting parameters and bioink composition, are discussed.
• Distinct bioprinting approaches are identified for undifferentiated, adult stem cell, and terminally differentiated iPSC-derived cells.

Conclusions:

• 3D bioprinting of iPSC represents a transformative approach in regenerative medicine.
• Optimized bioprinting strategies are crucial for successful tissue engineering using iPSC.
• Further research can advance patient-specific therapies through iPSC-based bioprinting.