Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Elements and Compounds01:27

Elements and Compounds

103.3K
Pure substances consist of only one type of matter. A pure substance can be an element or a compound. An element consists of only one type of atom, while a compound consists of two or more types of atoms held together by a chemical bond.
Elements
Elements are classified as atomic or molecular based on the nature of their basic units. They are unique forms of matter with specific chemical and physical properties that cannot break down into smaller substances by ordinary chemical reactions. There...
103.3K
Periodic Classification of the Elements04:00

Periodic Classification of the Elements

58.7K
The periodic table arranges atoms based on increasing atomic number so that elements with the same chemical properties recur periodically. When their electron configurations are added to the table, a periodic recurrence of similar electron configurations in the outer shells of these elements is observed. Because they are in the outer shells of an atom, valence electrons play the most important role in chemical reactions. The outer electrons have the highest energy of the electrons in an atom...
58.7K
Key Elements for Plant Nutrition02:35

Key Elements for Plant Nutrition

24.0K
Like all living organisms, plants require organic and inorganic nutrients to survive, reproduce, grow and maintain homeostasis. To identify nutrients that are essential for plant functioning, researchers have leveraged a technique called hydroponics. In hydroponic culture systems, plants are grown—without soil—in water-based solutions containing nutrients. At least 17 nutrients have been identified as essential elements required by plants. Plants acquire these elements from the...
24.0K
Classification of Elements and Compounds02:54

Classification of Elements and Compounds

72.8K
Pure substances consist of only one type of matter. A pure substance can be an element or a compound. An element consists of only one type of atom, while a compound consists of two or more types of atoms held together by a chemical bond. Elements are classified as atomic or molecular based on the nature of their basic units.
Compounds are pure substances composed of two or more elements in fixed, definite proportions. Compounds are classified as ionic or molecular (covalent) based on the bonds...
72.8K
Defenses Against Pathogens and Herbivores02:26

Defenses Against Pathogens and Herbivores

29.5K
Plants present a rich source of nutrients for many organisms, making it a target for herbivores and infectious agents. Plants, though lacking a proper immune system, have developed an array of constitutive and inducible defenses to fend off these attacks.
29.5K
Defense Against Bacterial Pathogens01:31

Defense Against Bacterial Pathogens

2.7K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
2.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Functional CRISPR screens reveal TPL1 lncRNA as a regulator of triple-negative breast cancer hallmarks.

Journal of advanced research·2026
Same author

Rapid Phenotypic Drug Susceptibility Testing of <i>Mycobacterium tuberculosis</i> Using Raman-Deuterium Isotope Probing.

Analytical chemistry·2026
Same author

Circulating Inflammation-Related Proteins Linked to Corneal Neuroimmune Measures, Neuropathy Deficits, and Symptoms in Type 2 Diabetes.

Journal of the peripheral nervous system : JPNS·2026
Same author

Integration of substrate-specific enzymes and a peroxide biosensor for detection of glucose, uric acid, and cholesterol.

Applied and environmental microbiology·2026
Same author

From the Proteome to Therapeutics: A Multi-Database Approach to Drug Discovery in Periodontitis-An Exploratory Pilot Study.

Journal of clinical periodontology·2026
Same author

Diversity-generating retroelements for programmable targeted hypermutagenesis.

Nature biotechnology·2026
Same journal

Vision and Development of a Design, Implementation, and Verification Automation (DIVA) Software Platform for DNA Construction.

ACS synthetic biology·2026
Same journal

Engineering a Cytochrome P450 <i>O</i>-Demethylase for the Bioconversion of Hardwood Lignin.

ACS synthetic biology·2026
Same journal

Genetic Biosensor for Optimizing Double-Stranded RNA Production by Bacteria.

ACS synthetic biology·2026
Same journal

Heterologous Expression of an Abandoned Termite Mound Fungus Gene Cluster Reveals a Protective Aldehyde-Alcohol Cycle and a Candidate Termiticidal Metabolite.

ACS synthetic biology·2026
Same journal

A Framework for the In Vivo Production of Extensively Engineered Thiopeptides.

ACS synthetic biology·2026
Same journal

A Highly Stringent Split Intein-Mediated DHFR Selectable Marker Enables Efficient Development of High-Producing CHO Cells for Therapeutic Proteins.

ACS synthetic biology·2026
See all related articles

Related Experiment Video

Updated: Jan 21, 2026

Characterization and Functional Prediction of Bacteria in Ovarian Tissues
10:12

Characterization and Functional Prediction of Bacteria in Ovarian Tissues

Published on: October 23, 2021

3.2K

Defensive Function of Transposable Elements in Bacteria.

Catherine Fan1, Yin-Hu Wu1,2, Christoph M Decker3

  • 1Department of Engineering Science , University of Oxford , Parks Road , OX1 3PJ Oxford , United Kingdom.

ACS Synthetic Biology
|August 4, 2019
PubMed
Summary
This summary is machine-generated.

Insertion sequences (ISs) in Escherichia coli can protect host cells by disrupting harmful genes. This disruption restores cell fitness, offering insights into gene circuit stability in synthetic biology.

Keywords:
Escherichia coliIS1IS10insertion sequenceproteomicsstresssynthetic biologytransposable elements

More Related Videos

The Ex Vivo Colon Organ Culture and Its Use in Antimicrobial Host Defense Studies
10:40

The Ex Vivo Colon Organ Culture and Its Use in Antimicrobial Host Defense Studies

Published on: February 13, 2017

14.8K
Systemic Bacterial Infection and Immune Defense Phenotypes in Drosophila Melanogaster
10:12

Systemic Bacterial Infection and Immune Defense Phenotypes in Drosophila Melanogaster

Published on: May 13, 2015

25.3K

Related Experiment Videos

Last Updated: Jan 21, 2026

Characterization and Functional Prediction of Bacteria in Ovarian Tissues
10:12

Characterization and Functional Prediction of Bacteria in Ovarian Tissues

Published on: October 23, 2021

3.2K
The Ex Vivo Colon Organ Culture and Its Use in Antimicrobial Host Defense Studies
10:40

The Ex Vivo Colon Organ Culture and Its Use in Antimicrobial Host Defense Studies

Published on: February 13, 2017

14.8K
Systemic Bacterial Infection and Immune Defense Phenotypes in Drosophila Melanogaster
10:12

Systemic Bacterial Infection and Immune Defense Phenotypes in Drosophila Melanogaster

Published on: May 13, 2015

25.3K

Area of Science:

  • Microbiology
  • Genetics
  • Synthetic Biology

Background:

  • Transposable elements, like insertion sequences (ISs), have debated effects on host cells.
  • Understanding their role is crucial for applications like synthetic biology.

Purpose of the Study:

  • To investigate the role of ISs in Escherichia coli.
  • To determine if ISs can provide a defensive function for the host cell.

Main Methods:

  • Utilized three E. coli strains (S17, DH5α, Nissle 1917).
  • Introduced IS1 and IS10 into the I-CeuI gene on the pLO11-ICeuI plasmid.
  • Performed proteomics analysis at different generations (1st, 5th, 31st).

Main Results:

  • IS1 and IS10 rapidly disrupted the I-CeuI gene across all tested E. coli strains.
  • Leaky I-CeuI expression induced DNA damage, triggering SOS response and altering cellular processes.
  • Post-disruption, E. coli DH5α showed recovery in protein profiles by the 31st generation.

Conclusions:

  • Insertion sequences can act defensively by mutating harmful genes, restoring host fitness.
  • This mechanism has significant implications for the stability of engineered gene circuits in synthetic biology.