Related Experiment Video

Updated: Jan 21, 2026

The Sciatic Nerve Cuffing Model of Neuropathic Pain in Mice
07:09

The Sciatic Nerve Cuffing Model of Neuropathic Pain in Mice

Published on: July 16, 2014

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Effects of miR-150 on neuropathic pain process via targeting AKT3

Wei Cai1, Yang Zhang2, Yan Liu2

  • 1Department of Orthopedics, The Affiliated Huai'an NO.1 People's Hospital of Nanjing Medical University, Huai'an Jiangsu, China.

Biochemical and Biophysical Research Communications
|August 5, 2019
PubMed

Insights

MicroRNAs (miRNAs) can alleviate neuropathic pain. Our study shows miR-150 targets AKT3 to reduce pain in a rat model, suggesting miR-150 as a therapeutic target for neuropathic pain.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Neuropathic pain is a debilitating condition with limited treatment options.
  • MicroRNAs (miRNAs) are implicated in pain regulation, but their specific roles remain unclear.
  • Understanding miRNA mechanisms is crucial for developing novel pain therapies.

Purpose of the Study:

  • To investigate the role of miR-150 in neuropathic pain development.
  • To identify molecular targets of miR-150 in the context of pain.
  • To explore the therapeutic potential of miR-150 for neuropathic pain.

Main Methods:

  • A rat model of neuropathic pain was established using chronic sciatic nerve injury (CCI).
  • Bioinformatic analysis was used to predict potential miRNA targets.
  • miR-150 expression levels and its target AKT3 (mRNA and protein) were quantified in vivo.
  • Pain behaviors (mechanical and thermal hyperalgesia) were assessed.

Main Results:

  • Increased miR-150 expression significantly alleviated neuropathic pain in CCI rats.
  • AKT3 was identified as a direct target of miR-150.
  • Overexpression of miR-150 repressed AKT3 expression and reduced pain sensitivity.
  • The pain-alleviating effects of miR-150 were reversed by AKT3 overexpression.

Conclusions:

  • miR-150 restrains neuropathic pain by targeting AKT3 in vivo.
  • This study highlights miR-150 as a potential therapeutic target for neuropathic pain.
  • Targeting the miR-150/AKT3 pathway offers a promising strategy for pain management.
Keywords:
AKT3Neuropathic painmicroRNA-150

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