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Updated: Jan 21, 2026

Ultra-long Read Sequencing for Whole Genomic DNA Analysis
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Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

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Complete genome sequence and phylogenetic analysis of a novel aquareovirus isolated from a diseased marbled eel

Hui-Wen Huang1, Chiao-Hsuan Huang1, Chiu-Ming Wen2

  • 1Department of Life Sciences, National University of Kaohsiung, No. 700, Kaohsiung University Road, Nan-Tzu District, Kaohsiung, 81148, Taiwan.

Archives of Virology
|August 5, 2019
PubMed

Insights

A novel Marbled eel reovirus (MERV) was identified in diseased eels. This aquareovirus (AQRV) shows unique genomic and protein sequences, suggesting a new species, "Aquareovirus H".

Area of Science:

  • Virology
  • Aquatic Animal Health
  • Molecular Biology

Background:

  • Marbled eel reovirus (MERV) is an aquareovirus (AQRV) linked to diseased marbled eels exhibiting skin hemorrhage.
  • Aquareoviruses are known pathogens affecting aquatic organisms.

Purpose of the Study:

  • To characterize the novel Marbled eel reovirus (MERV) isolated from diseased marbled eels.
  • To determine the genomic and phylogenetic relationships of MERV within the Aquareovirus genus.

Main Methods:

  • Viral propagation in Aequidens rivulatus brain cell line.
  • Purification of viral particles using cesium chloride gradient.
  • Next-generation sequencing for genomic RNA analysis.
  • Phylogenetic analysis based on structural protein sequences.

Main Results:

  • MERV possesses 11 double-stranded RNA segments encoding 12 proteins, typical of AQRVs.
  • MERV genome sequences show minimal similarity to known AQRV strains.
  • Structural proteins are most related to American grass carp reovirus (≤64.89% identity).
  • Phylogenetic analysis places MERV evolutionarily between AQRV-B and -G subgroups.
  • MERV shows closer relationships to orthoreoviruses based on NS38 and NS73 protein sequences.

Conclusions:

  • MERV represents a novel aquareovirus, potentially a new species named "Aquareovirus H".
  • The findings highlight the need for further investigation into AQRV taxonomy and evolution.
  • This research contributes to understanding viral diversity in aquatic environments.

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