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LncRNA CASC11 promotes the development of lung cancer through targeting microRNA-302/CDK1 axis

W Tong1, T-C Han, W Wang

  • 1Department of Thoracic Surgery, Cancer Hospital of China Medical University, Liaoning Cancer Hospital and Institute, Shenyang, China. tianci2010@vip.qq.com.

European Review for Medical and Pharmacological Sciences
|August 6, 2019
PubMed
Abstract

Insights

High expression of long non-coding RNA CASC11 promotes lung cancer by increasing CDK1 levels via microRNA-302. This study reveals a novel mechanism in lung cancer development.

Area of Science:

  • Molecular Biology
  • Oncology
  • Gene Regulation

Background:

  • Lung cancer is a leading cause of cancer-related mortality worldwide.
  • Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in cancer development.
  • The specific role of lncRNA CASC11 in lung cancer pathogenesis remains to be fully elucidated.

Purpose of the Study:

  • To investigate the role of lncRNA CASC11 in lung cancer development.
  • To determine if CASC11 targets the microRNA-302/CDK1 axis in lung cancer.
  • To elucidate the underlying molecular mechanisms of CASC11 in lung cancer progression.

Main Methods:

  • Quantitative real-time polymerase chain reaction (qRT-PCR) to measure expression levels of CASC11, microRNA-302, and CDK1.
  • Cell counting kit-8 (CCK-8) and colony formation assays to assess cell proliferation.
  • Dual-luciferase reporter gene assay to confirm binding interactions between CASC11, microRNA-302, and CDK1.
  • Western blot analysis to detect protein expression levels.

Main Results:

  • lncRNA CASC11 and CDK1 were significantly upregulated, while microRNA-302 was downregulated in lung cancer tissues and cell lines.
  • CASC11 knockdown inhibited lung cancer cell proliferation, whereas microRNA-302 knockdown promoted it.
  • Dual-luciferase assays confirmed that CASC11 directly binds to microRNA-302, and microRNA-302 directly binds to CDK1.
  • CASC11 promotes lung cancer cell proliferation by upregulating CDK1 expression through sponging microRNA-302.

Conclusions:

  • lncRNA CASC11 plays a crucial role in promoting lung cancer development.
  • The CASC11/microRNA-302/CDK1 axis represents a potential therapeutic target for lung cancer.
  • Understanding this regulatory pathway provides insights into the molecular mechanisms of lung cancer.

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