Related Experiment Video
Updated: Jan 21, 2026
Detection of Targetable Alterations in Non-small Cell Lung Cancer using Next-generation Sequencing
Published on: October 10, 2025
Hsa-miR-337 inhibits non-small cell lung cancer cell invasion and migration by targeting TCF7
1Department of Oncology, The First Affiliated Hospital of Dalian Medical University, Dalian, China. dashun1210@163.com.
Objective:
Recent studies have revealed that microRNAs (miRNAs) play a crucial role in the progression of tumorigenesis. Non-small cell lung cancer (NSCLC) is one of the most common malignancies worldwide. The aim of this study was to identify the exact role of hsa-miR-337 in the progression of NSCLC and to investigate the possible underlying mechanism.
Patients And Methods:
Hsa-miR-337 expression in NSCLC cells and 60 paired tissue samples were detected by Real Time-quantitative Polymerase Chain Reaction (RT-qPCR). Moreover, the functions of hsa-miR-337 in vitro were identified by transwell assay and wound healing assay, respectively. Furthermore, the underlying mechanism was explored by RT-qPCR, Western blot assay, and luciferase assay.
Results:
The expression level of hsa-miR-337 in NSCLC tissues was remarkably down-regulated when compared with that of adjacent normal samples. Moreover, the invasion and migration of NSCLC cells were significantly inhibited after overexpression of hsa-miR-337 in vitro. Moreover, after overexpression of hsa-miR-337 in vitro, the mRNA and protein levels of TCF7 were significantly down-regulated. Besides, the expression of TCF7 in NSCLC tissues was negatively correlated with the expression of hsa-miR-337.
Conclusions:
The above results suggested that hsa-miR-337 could repress the migration and invasion of NSCLC cells through directly targeting TCF7. Furthermore, hsa-miR-337 might offer a new therapeutic intervention for NSCLC patients.
Insights
Hsa-miR-337 is down-regulated in non-small cell lung cancer (NSCLC). Overexpressing hsa-miR-337 inhibits NSCLC cell migration and invasion by targeting TCF7, suggesting therapeutic potential.
Area of Science:
- Oncology
- Molecular Biology
- Gene Regulation
Background:
- MicroRNAs (miRNAs) are critical regulators in tumorigenesis.
- Non-small cell lung cancer (NSCLC) is a prevalent global malignancy.
- Understanding specific miRNA roles in NSCLC is vital for therapeutic development.
Purpose of the Study:
- To determine the role of hsa-miR-337 in NSCLC progression.
- To elucidate the molecular mechanism by which hsa-miR-337 influences NSCLC.
Main Methods:
- Real-Time quantitative Polymerase Chain Reaction (RT-qPCR) for expression analysis.
- Transwell and wound healing assays to assess cell migration and invasion.
- Western blot and luciferase assays to investigate molecular targets and pathways.
Main Results:
- Hsa-miR-337 expression was significantly reduced in NSCLC tissues compared to normal adjacent tissues.
- Overexpression of hsa-miR-337 suppressed NSCLC cell invasion and migration in vitro.
- TCF7 mRNA and protein levels were downregulated upon hsa-miR-337 overexpression, with an inverse correlation in patient tissues.
Conclusions:
- Hsa-miR-337 inhibits NSCLC cell migration and invasion by directly targeting TCF7.
- Hsa-miR-337 presents a potential novel therapeutic target for NSCLC treatment.
Related Concept Videos
01:35Cancer Cell Migration through Invadopodia
01:19Cell Migration
01:09Cell Migration
01:21Chemotaxis and Direction of Cell Migration
01:18Role of Myosin in Cell Migration
Myosin II is a hexamer comprising two heavy chains with globular heads and coiled-coil tails, two regulatory light chains, and two essential light chains. The ATPase sites on the myosin heads hydrolyze ATP, and the released phosphate generates the force for contraction....
01:32Cytoskeletal Coordination in Cell Migration