A quantitative analysis of carbon-ion beam-induced reactive oxygen species and redox reactions

The amounts of reactive oxygen species generated in aqueous samples by irradiation with X-ray or clinical carbon-ion beams were quantified. Hydroxyl radical (^•OH), hydrogen peroxide (H₂O₂), and the total amount of oxidation reactions, which occurred mainly because of ^•OH and/or hydroperoxy radicals (HO₂ ^•), were measured by electron paramagnetic resonance-based methods. ^•OH generation was expected to be localized on the track/range of the carbon-ion beam/X-ray, and mM and M levels of ^•OH generation were observed. Total ^•OH generation levels were identical at the same dose irrespective of whether X-ray or carbon-ion beam irradiation was used, and were around 0.28-0.35 µmol/L/Gy. However, sparse ^•OH generation levels decreased with increasing linear energy transfer, and were 0.17, 0.15, and 0.09 µmol/L/Gy for X-ray, 20 keV/µm carbon-ion beam, and >100 keV/µm carbon-ion beam sources, respectively. H₂O₂ generation was estimated as 0.26, 0.20, and 0.17 µmol/L/Gy, for X-ray, 20 keV/µm carbon-ion beam, and >100 keV/µm carbon-ion beam sources, respectively, whereas the ratios of H₂O₂ generation per oxygen consumption were 0.63, 0.51, and 3.40, respectively. The amounts of total oxidation reactions were 2.74, 1.17, and 0.66 µmol/L/Gy, respectively. The generation of reactive oxygen species was not uniform at the molecular level.