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Updated: Jan 21, 2026
09:36
Optical Imaging of Neurons in the Crab Stomatogastric Ganglion with Voltage-sensitive Dyes
Published on: March 23, 2011
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Primer to Voltage Imaging With ANNINE Dyes and Two-Photon Microscopy
Bernd Kuhn1, Christopher J Roome1
1Okinawa Institute of Science and Technology Graduate University, Okinawa, Japan.
Frontiers in Cellular Neuroscience
|August 6, 2019
Summary
ANNINE voltage-sensitive dyes enable precise in vivo neuronal voltage recording with two-photon microscopy. These dyes offer high sensitivity and minimal phototoxicity for advanced neuroscience research.
Area of Science:
- Neuroscience
- Biophysics
- Optical Imaging
Background:
- Voltage-sensitive dyes are crucial for monitoring neuronal activity.
- Fast voltage-sensitive dyes are needed for high-resolution recordings.
- ANNINE dyes offer unique advantages for voltage imaging.
Purpose of the Study:
- To detail the voltage sensing mechanism of ANNINE dyes.
- To explain optimization strategies for voltage imaging.
- To highlight the utility of ANNINE dyes for in vivo neuroscience.
Main Methods:
- Utilized two-photon microscopy for voltage imaging.
- Applied ANNINE-6 and ANNINE-6plus dyes for neuronal recording.
- Performed voltage imaging in bulk loaded tissue and single neurons.
Main Results:
- ANNINE dyes exhibit high sensitivity (0.5%/mV), low bleaching, and phototoxicity.
- Achieved nanosecond temporal resolution, exceeding current imaging capabilities.
- Demonstrated stable in vivo labeling for up to two weeks.
Conclusions:
- ANNINE dyes are ideal for in vivo neuronal voltage recording.
- Two-photon microscopy with ANNINE dyes allows depth-resolved and single-neuron imaging.
- Integration with other techniques enhances neuroscience research potential.
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