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Related Experiment Videos

Intermediate filaments in mouse taste bud cells.

M Takeda1, N Obara, Y Suzuki

  • 1Department of Anatomy, Higashi Nippon Gakuen University School of Dentistry, Hokkaido, Japan.

Archives of Histology and Cytology
|March 1, 1988
PubMed
Summary
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Mouse taste bud cells contain keratins, similar to surrounding epithelial cells, but with distinct subtypes. This suggests taste bud cells originate from epithelial cells and undergo keratin changes during differentiation.

Area of Science:

  • Cell Biology
  • Histology
  • Molecular Biology

Background:

  • Intermediate filaments, particularly keratins, are crucial structural components in epithelial tissues.
  • Taste buds are complex sensory organs composed of various cell types embedded within surrounding epithelium.

Purpose of the Study:

  • To investigate the presence and distribution of intermediate filaments, specifically keratins, in mouse taste bud cells.
  • To compare keratin expression and intermediate filament organization between taste bud cells and surrounding epithelial cells.
  • To explore the potential origin and differentiation pathways of taste bud cells based on keratin profiles.

Main Methods:

  • Immunocytochemistry using anti-keratin antibodies.
  • Conventional electron microscopy to examine intermediate filament aggregation.

Related Experiment Videos

  • Differential antibody staining (e.g., PKK2) to identify specific keratin subtypes.

    • Main Results:

    • Taste bud cells (Types I, II, III) exhibit less dense intermediate filament bundles than surrounding epithelial cells.
    • Type III taste bud cells show denser filament aggregation compared to Type I and II cells.
    • Keratins are present in all taste bud and surrounding epithelial cells, but specific keratin subtypes differ, with PKK2 antibody only staining surrounding epithelial cells.

    Conclusions:

    • Keratin expression differs between taste bud cells and surrounding epithelial cells, indicating distinct subtypes are present.
    • The findings support the hypothesis that all taste bud cell types, including Type III receptor cells, originate from the surrounding epithelial cells.
    • Changes in keratin subtypes and intermediate filament organization patterns occur during the differentiation process from epithelial cells to taste bud cells.