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Identification of the Potential Key Long Non-coding RNAs in Aged Mice With Postoperative Cognitive Dysfunction.

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|August 6, 2019
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Summary
This summary is machine-generated.

This study identifies key long non-coding RNAs (lncRNAs) involved in postoperative cognitive dysfunction (POCD). These lncRNAs are linked to neuronal inflammation and apoptosis, offering potential therapeutic targets for POCD.

Keywords:
agingapoptosisinflammationlong non-coding RNAmicroarraypostoperative cognitive dysfunction

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Postoperative cognitive dysfunction (POCD) is a common complication, especially in elderly patients after surgery.
  • Long non-coding RNAs (lncRNAs) are increasingly recognized for their role in the pathogenesis of neurological disorders, including POCD.

Purpose of the Study:

  • To identify key lncRNAs and their target messenger RNAs (mRNAs) implicated in the development of POCD.
  • To explore the functional roles of these dysregulated genes in the context of surgery-induced brain changes.

Main Methods:

  • Microarray analysis of lncRNA and mRNA expression profiles in hippocampal tissues from POCD and control mice.
  • Gene Ontology (GO) and KEGG pathway enrichment analyses to understand gene functions.
  • Construction of coding-non-coding co-expression (CNC) networks to identify lncRNA-mRNA interactions.

Main Results:

  • Identified 868 differentially expressed lncRNAs and 690 differentially expressed mRNAs.
  • Dysregulated genes were primarily associated with inflammatory and apoptotic signaling pathways.
  • Specific lncRNAs (uc009qbj.1, ENSMUST00000174338, NONMMUT00000123687) were correlated with genes involved in inflammation and apoptosis, such as Vrk2, Smad7, and Meis2.

Conclusions:

  • Several lncRNAs play a significant role in POCD pathogenesis by regulating neuronal inflammation and apoptosis.
  • These identified lncRNAs and their target mRNAs represent potential biomarkers and therapeutic targets for POCD.