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Related Experiment Videos

A developmentally regulated lectin in Bufo arenarum embryos.

M T Elola1, N E Fink-de-Cabutti, H Herkovits

  • 1Instituto de Biología de la Reproducción y Desarrollo Embrionario, Universidad Nacional de Lomas de Zamora, Argentina.

Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Medicas E Biologicas
|January 1, 1987
PubMed
Summary

Bufo arenarum embryos exhibit beta-galactoside lectin activity that changes during development. This lectin

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Area of Science:

  • Developmental Biology
  • Biochemistry
  • Molecular Biology

Background:

  • Endogenous lectins play roles in biological processes.
  • Beta-galactoside lectins are involved in cell recognition.

Purpose of the Study:

  • To characterize the activity and saccharide inhibition of beta-galactoside lectin in Bufo arenarum embryos.
  • To investigate developmental changes in lectin activity and specificity.

Main Methods:

  • Extracting lectin activity from Bufo arenarum whole embryos.
  • Measuring lectin activity using trypsinized rabbit red blood cells.
  • Assessing inhibition by various saccharides at different developmental stages.

Main Results:

  • Lectin activity was high and fluctuating in early embryonic stages (up to 76 hpf), decreasing significantly in later stages (77-264 hpf).

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  • Lactose was the most potent inhibitor, while alpha-galactoside saccharides showed weaker inhibition.
  • The lectin exhibited altered sugar specificity at the final embryonic stage.

    • Conclusions:

    • Bufo arenaruem embryonic lectin activity changes dynamically during development.
    • The observed changes suggest lectin modification or synthesis of new lectins.
    • These lectins may be involved in polyspermy blockage or embryonic cell-cell interactions.