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Cell-Free Scaled Production and Adjuvant Addition to a Recombinant Major Outer Membrane Protein from Chlamydia muridarum for Vaccine Development
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Effect of Recombinant Helicobacter Outer Membrane Protein H (HopH) on Nitric Oxide Production by Peripheral

Masoumeh Navidinia1, Neda Soleimani2, Narges Bodagh Abadi3

  • 1Faculty of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Avicenna Journal of Medical Biotechnology
|August 6, 2019
PubMed
Abstract

Insights

Recombinant HopH from Helicobacter pylori modulates nitric oxide production in mouse macrophages. High concentrations showed a toxic effect, suggesting potential as an anticancer candidate.

Area of Science:

  • Immunology
  • Microbiology

Background:

  • Bacterial products can act as immunomodulators.
  • Helicobacter pylori outer membrane proteins are key immune stimulators.

Purpose of the Study:

  • To investigate the in vitro effects of recombinant HopH from H. pylori on nitric oxide (NO) production and macrophage viability.

Main Methods:

  • Produced H. pylori recombinant HopH.
  • Isolated and cultured mouse peritoneal macrophages.
  • Assessed NO production using Griess reagent and cell viability via MTT assay after HopH stimulation.

Main Results:

  • Recombinant HopH exhibited a dose-dependent effect on NO release, with suppression at high concentrations.
  • A stimulatory effect was observed at 15 µg/ml.
  • Significant NO stimulation occurred in LPS-stimulated macrophages treated with HopH.

Conclusions:

  • Recombinant HopH demonstrates a concentration-dependent toxicity on macrophages.
  • These findings suggest recombinant HopH as a potential anticancer therapeutic agent.
Keywords:
Helicobacter pyloriMacrophagesRecombinant HopH

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