Related Experiment Video

Updated: Jan 21, 2026

Enrichment for Chemoresistant Ovarian Cancer Stem Cells from Human Cell Lines
11:50

Enrichment for Chemoresistant Ovarian Cancer Stem Cells from Human Cell Lines

Published on: September 10, 2014

13.9K

CHTOP in Chemoresistant Epithelial Ovarian Cancer: A Novel and Potential Therapeutic Target

Xiaojie Feng1,2,3, Xupeng Bai2,3, Jie Ni2,3

  • 1Department of Gynaecological Oncology, Henan Cancer Hospital, Zhengzhou, China.

Frontiers in Oncology
|August 6, 2019
PubMed

Insights

Chromatin target of protein arginine methyltransferase (CHTOP) is highly expressed in chemoresistant epithelial ovarian cancer (EOC). Reducing CHTOP enhances apoptosis and decreases stemness and metastasis, suggesting it as a therapeutic target for EOC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Chemoresistance presents a significant hurdle in treating epithelial ovarian cancer (EOC).
  • Chromatin target of protein arginine methyltransferase (CHTOP) was identified as a potential biomarker in chemoresistant EOC cell lines.
  • Understanding CHTOP's role is crucial for developing novel therapeutic strategies against EOC.

Purpose of the Study:

  • To investigate the role of CHTOP in chemoresistant EOC.
  • To elucidate the underlying mechanisms by which CHTOP influences chemoresistance.
  • To evaluate CHTOP as a potential therapeutic target for overcoming EOC chemoresistance.

Main Methods:

  • Detected CHTOP expression in ovarian cancer cells and tissues using immunofluorescence, western blot, and immunohistochemistry.
  • Assessed apoptosis, stemness, and metastasis in chemoresistant EOC cells following CHTOP knockdown (KD) using flow cytometry, TUNEL assay, colony/sphere formation assays, and invasion/wound-healing assays.
  • Utilized bioinformatic analysis to correlate CHTOP expression with patient survival.

Main Results:

  • CHTOP was significantly upregulated in chemoresistant EOC cells and human ovarian cancer tissues, correlating with poor progression-free survival.
  • CHTOP knockdown enhanced cisplatin-induced apoptosis in chemoresistant EOC cells.
  • CHTOP KD reduced cancer stemness and decreased the metastatic potential of chemoresistant EOC cells.

Conclusions:

  • CHTOP plays a critical role in promoting apoptosis resistance, stemness, and metastasis in chemoresistant EOC.
  • Targeting CHTOP presents a promising strategy to overcome chemoresistance in epithelial ovarian cancer.
  • CHTOP is a potential biomarker and therapeutic target for improving EOC treatment outcomes.
Keywords:
CHTOPapoptosischemoresistancemetastasisovarian cancerstemness

More Related Videos

In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer07:46

In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer

Published on: August 17, 2010

34.5K
Heterotypic Three-dimensional In Vitro Modeling of Stromal-Epithelial Interactions During Ovarian Cancer Initiation and Progression12:42

Heterotypic Three-dimensional In Vitro Modeling of Stromal-Epithelial Interactions During Ovarian Cancer Initiation and Progression

Published on: August 28, 2012

15.6K

Related Concept Videos

Targeted Cancer Therapies02:57

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
8.7K
Therapeutic Index01:13

Therapeutic Index

The therapeutic index of a drug is a key parameter in pharmacology that quantifies the relative safety of a drug by calculating the ratio between the dose that causes toxicity in half the population (50%) to the dose that proves to be effective for half the population (50%). It provides a spectrum of doses for a particular drug ranging from effective to potentially toxic. To illustrate, consider an anticoagulant agent like warfarin. It possesses a narrow window within its therapeutic index to...
6.7K
Ovarian Cycle01:27

Ovarian Cycle

The menstrual cycle includes a critical component known as the ovarian cycle, which undergoes two main phases each month—the follicular phase and the luteal phase. The follicular phase is variable and averaging around 14 days. Ovulation, triggered by a surge in luteinizing hormone (LH), marks the transition between the two phases. The second phase, the luteal phase, is relatively consistent, lasting approximately 14 days, and is marked by the activity of the corpus luteum. While a cycle...
3.5K
Potential Energy00:52

Potential Energy

The energy stored by a structure and location of matter in space is called potential energy. For instance, raising a kettlebell changes its spatial location and increases its potential energy. Similarly, a stretched rubber band contains potential energy which, under certain conditions, can be converted into other forms of energy, such as kinetic energy.
Chemical bonds that form attractive forces between atoms also contain potential energy, called chemical energy. When a chemical reaction...
42.3K
Standard Electrode Potentials03:02

Standard Electrode Potentials

On comparing the reactivity of silver and lead, it is observed that the two ionic species, Ag+ (aq) and Pb2+ (aq), show a difference in their redox reactivity towards copper: the silver ion undergoes spontaneous reduction, while the lead ion does not. This relative redox activity can be easily quantified in electrochemical cells by a property called cell potential. This property is commonly known as cell voltage in electrochemistry, and it is a measure of the energy which accompanies the charge...
50.0K
Therapeutic Communication01:30

Therapeutic Communication

Communication is a lifelong learning process. Through therapeutic communication, nurses can collect relevant assessment data, provide education and counseling, and interact during nursing interventions. Sending and receiving messages occur through verbal and nonverbal communication techniques and can happen separately or simultaneously.
Verbal communication depends on language or a prescribed way of using words so that people can share information effectively. The critical aspects of verbal...
7.6K