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Related Experiment Videos

Hemolysin from Listeria--biochemistry, genetics and function in pathogenesis.

W Goebel1, S Kathariou, M Kuhn

  • 1Institut für Genetik und Mikrobiologie, Universität Würzburg.

Infection
|January 1, 1988
PubMed
Summary
This summary is machine-generated.

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This study identifies thiol-activated hemolysins (listeriolysins) in virulent Listeria monocytogenes strains. It also characterizes a distinct hemolytic protein in L. monocytogenes, differentiating it from listeriolysin.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Immunology

Background:

  • Thiol-activated hemolysins, known as listeriolysins, are virulence factors produced by Listeria monocytogenes.
  • Understanding the specific hemolysins and their production in different Listeria species is crucial for pathogen identification and virulence assessment.

Purpose of the Study:

  • To purify and characterize thiol-activated hemolysins from Listeria monocytogenes and Listeria ivanovii.
  • To investigate the presence and variability of listeriolysin production in virulent and avirulent Listeria strains.
  • To identify and differentiate hemolytic factors produced by Listeria monocytogenes.

Main Methods:

  • Purification of hemolysins from Listeria species.
  • N-terminal amino acid sequencing of purified proteins.

Related Experiment Videos

  • Western blot analysis using specific antibodies (anti-listeriolysin and anti-streptolysin O).
  • Construction and screening of a Listeria monocytogenes gene bank in Escherichia coli.
  • Transposon mutagenesis (Tn916) to generate nonhemolytic mutants.

    • Main Results:

    • Virulent Listeria monocytogenes strains synthesize and secrete listeriolysin (58-59 kDa), with variable quantities observed.
    • No listeriolysin cross-reactivity was detected in Listeria innocua, Listeria welshimeri, Listeria grayi, Listeria murrayi, or the hemolytic Listeria seeligeri.
    • Two types of hemolytic clones were identified in a Listeria monocytogenes gene bank: one producing a 23 kDa protein (DTT-independent) and another producing a 61 kDa protein cross-reactive with anti-listeriolysin/streptolysin O antibodies.
    • Type I nonhemolytic mutants produced no extracellular protein cross-reactive with anti-listeriolysin/SLO antibodies.

    Conclusions:

    • Virulent Listeria monocytogenes consistently produces listeriolysin, but production levels vary significantly.
    • Other Listeria species tested do not produce proteins cross-reactive with listeriolysin or streptolysin O antibodies.
    • A distinct hemolytic protein (23 kDa) exists in Listeria monocytogenes, separate from the thiol-activated listeriolysin.