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Updated: Jan 21, 2026

Normothermic Ex Vivo Kidney Perfusion for the Preservation of Kidney Grafts prior to Transplantation
08:12

Normothermic Ex Vivo Kidney Perfusion for the Preservation of Kidney Grafts prior to Transplantation

Published on: July 15, 2015

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Microbiological epidemiology of preservation fluids in transplanted kidney: a nationwide retrospective observational

A Corbel1, M Ladrière1, N Le Berre1

  • 1Nephrology Dialysis Transplantation Department, University of Lorraine, CHRU-Nancy, Nancy, France.

Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases
|August 6, 2019
PubMed
Abstract

Insights

Positive kidney transplant preservation fluid (PF) cultures are common, especially from deceased donors, often due to contamination during procurement. This highlights the need for improved donor management to reduce infection risks for recipients.

Area of Science:

  • Transplantation immunology
  • Microbiology
  • Infectious diseases

Background:

  • Kidney transplant recipients face high risks of donor-derived infections early post-transplant.
  • Preservation fluid (PF) cultures are used for microbiological analysis, but the risk of positive cultures is not well understood.
  • There is no established consensus on prophylactic antibiotic use following positive PF cultures.

Purpose of the Study:

  • To determine the epidemiology of bacterial and fungal agents in kidney transplant PF cultures.
  • To identify risk factors associated with positive PF cultures in kidney transplantation.
  • To inform clinical practice regarding donor-derived infections in kidney transplant recipients.

Main Methods:

  • Retrospective observational study using a national database.
  • Data collected from October 2015 to December 2016.
  • Analysis included donor, recipient, transplantation characteristics, donor infections, and PF microbiological data.

Main Results:

  • 20.5% of kidney transplants had positive PF cultures, with higher rates in deceased donors (24.1%) vs. living donors (1.8%).
  • Coagulase-negative staphylococci and Enterobacteriaceae were the most frequent microorganisms; 59.9% of positive cultures showed polymicrobial contamination.
  • Risk factors for positive PF cultures in deceased-donor transplants included intestinal perforation, multiorgan procurement, and en bloc transplantation; perfusion pump use and donor antibiotics reduced risk.

Conclusions:

  • Approximately 24% of deceased-donor PF cultures were positive, suggesting procurement contamination as a primary cause.
  • Understanding these epidemiological factors is crucial for managing infection risk in kidney transplant recipients.
  • Further research may refine strategies for preventing donor-derived infections.
Keywords:
Infectious riskKidney transplantationMicrobiological analysisOrgan procurement procedurePreservation fluid

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