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Modeling Breast Cancer in Human Breast Tissue using a Microphysiological System
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An antisense transcript mediates MALAT1 response in human breast cancer

Carla Pereira Gomes1, Sandrina Nóbrega-Pereira1,2, Beatriz Domingues-Silva1

  • 1Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, 1649-028, Lisbon, Portugal.

BMC Cancer
|August 7, 2019
PubMed
Abstract

Insights

The antisense transcript TALAM1 regulates and synergizes with MALAT1, a long non-coding RNA (lncRNA), impacting breast cancer cell migration and aggressiveness. This discovery reveals new therapeutic targets for cancer treatment.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cancer Research

Background:

  • Long non-coding RNAs (lncRNAs) are key regulators with cell-type specificity, offering therapeutic potential in aging and disease.
  • MALAT1, a conserved lncRNA in metastatic lung cancer, shows promise for cancer regression, but its regulation remains unclear.
  • The MALAT1 locus is associated with an antisense transcript, TALAM1, suggesting a regulatory relationship.

Purpose of the Study:

  • To characterize the expression dynamics and function of TALAM1 in relation to MALAT1.
  • To investigate the role of the TALAM1/MALAT1 pair in breast cancer tumorigenesis and aggressiveness.
  • To explore potential therapeutic strategies targeting the MALAT1 locus.

Main Methods:

  • Utilized a panel of breast cancer cell lines for in vitro studies.
  • Performed in vitro and in vivo migration assays to assess cell motility.
  • Analyzed the expression and functional interplay between TALAM1 and MALAT1.

Main Results:

  • Down-regulation of TALAM1 significantly impaired breast cancer cell migration in vitro and metastasis in vivo.
  • TALAM1 was demonstrated to cooperate with MALAT1 in regulating breast cancer aggressiveness and malignancy.
  • The study uncovered the complex regulation of the MALAT1 locus by its antisense partner, TALAM1.

Conclusions:

  • The sense/antisense pair TALAM1 and MALAT1 plays a crucial role in breast cancer progression.
  • TALAM1 acts as a regulator and synergizes with MALAT1, influencing key aspects of tumorigenesis.
  • This research identifies novel therapeutic targets within the MALAT1 locus for cancer intervention.
Keywords:
Breast cancerMALAT1MigrationTALAM1lncRNAs

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