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MTOR involved in bacterial elimination against Trueperella pyogenes infection based on mice model by transcriptome

Ting Huang1, Kai Cui2, Xuhao Song2

  • 1Antibiotics Research and Re-evaluation Key Laboratory of Sichuan Province, SichuanIndustrial Institute of Antibiotics, Chengdu University, Chengdu, China; Key Laboratory of Bio-resources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan University, Chengdu, China.

Veterinary Microbiology
|August 7, 2019
PubMed

Insights

Trueperella pyogenes infection up-regulates mechanistic target of rapamycin (mTOR), inhibiting autophagy. Inhibiting mTOR boosts autophagy, reducing bacterial load and protecting mice, revealing mTOR as a therapeutic target for T. pyogenes infections.

Area of Science:

  • Microbiology
  • Immunology
  • Molecular Biology

Background:

  • Trueperella pyogenes is an opportunistic pathogen causing suppurative lesions in livestock.
  • Understanding T. pyogenes infection mechanisms is crucial for developing treatments.

Purpose of the Study:

  • To elucidate the molecular mechanisms of T. pyogenes infection using transcriptomic analysis.
  • To investigate the role of mechanistic target of rapamycin (mTOR) in T. pyogenes pathogenesis and host defense.

Main Methods:

  • Large-scale transcriptome sequencing (RNA-sequencing) of infected mouse livers.
  • Analysis of gene expression changes, focusing on autophagy regulators like mTOR.
  • Experimental manipulation of mTOR and autophagy pathways in macrophages and mice.

Main Results:

  • T. pyogenes infection significantly up-regulated mTOR expression in mouse livers.
  • mTOR up-regulation inhibited host cell autophagy, aiding bacterial survival.
  • Inhibition of mTOR promoted autophagy, reduced T. pyogenes viability, and protected mice from infection.

Conclusions:

  • mTOR is a key regulator of autophagy-mediated elimination of T. pyogenes.
  • Targeting the mTOR-autophagy pathway offers a potential therapeutic strategy against T. pyogenes infections.
Keywords:
Bacterial eliminationDifferentially expressed genesTranscriptomeTrueperella pyogenesmTOR autophagy

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