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Related Concept Videos

Quality Control01:05

Quality Control

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Quality control is one of the three cyclical quality assurance activities that help keep a system under statistical control. Typical quality control activities include creating quality control charts, conducting proficiency testing, and documenting and archiving results.
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Vasectomy is a surgical form of male sterilization that involves severing and sealing the vasa deferentia, preventing sperm from mixing with semen during ejaculation. Because a vasectomy does not impact the testes' ability to produce testosterone, hormone levels, libido, and sexual function generally remain unchanged. While vasectomy is highly effective in preventing pregnancy, with a success rate near 99.85%, rare cases of recanalization (spontaneous reconnection) can occur. Although...
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Microbial growth control refers to various methods employed to inhibit, reduce, or eliminate microorganisms to ensure safety and hygiene across different settings. These methods are categorized based on the target environment and the level of microbial control required.Biocides are versatile agents designed to control microorganisms by either inhibiting their growth or outright killing them. These agents work through various physical, chemical, mechanical, or biological mechanisms. The...
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Biological agents offer an effective means of controlling microbial growth by leveraging natural processes like predation, competition, and the secretion of antimicrobial substances.Predatory bacteria such as Bdellovibrio species target and kill pathogens like Salmonella and E. coli. They are widely used in poultry farms to control infections. Myxococcus species help combat plant-pathogenic fungi. These naturally occurring predators serve as eco-friendly alternatives to chemical pesticides and...
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Controlled-Potential Coulometry: Electrolytic Methods01:17

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Controlled-potential coulometry, also known as potentiostatic coulometry, employs a three-electrode system in which the working electrode's potential is precisely regulated using a potentiostat. Platinum working electrodes are utilized for positive potentials, while mercury pool electrodes are favored for extremely negative potentials. The platinum counter electrode is separated from the analyte using a membrane or salt bridge to avoid interference in the analysis.
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Quality of Water01:19

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In concrete preparation, the quality of water is paramount as it affects the strength and durability of the concrete. Potable water is usually preferred; however, it must not have excessive sodium or potassium to prevent compromising the concrete's integrity. Water quality is typically evaluated based on impurities such as dissolved solids, chlorides, and sulfates, and its pH value is ideally between 6 and 8. Even slightly acidic natural water may be acceptable unless it contains harmful...
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Sequencing of mRNA from Whole Blood using Nanopore Sequencing
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Versatile Quality Control Methods for Nanopore Sequencing.

Davide Bolognini1,2, Roberto Semeraro1, Alberto Magi3

  • 1Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy.

Evolutionary Bioinformatics Online
|August 7, 2019
PubMed
Summary
This summary is machine-generated.

Assessing nanopore sequencing data quality is crucial for biological discovery. We present NanoR, a quality control tool, and two pipelines (NanoR and PyPore) for reliable nanopore data analysis.

Keywords:
NGSanoporequality control

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Area of Science:

  • Genomics
  • Bioinformatics
  • Molecular Biology

Background:

  • Third-generation sequencing technologies, particularly nanopore sequencing, offer advantages over next-generation sequencing.
  • Ensuring the quality of nanopore sequencing data is critical for its reliable application in biological research.
  • Existing quality control methods require evaluation in the context of novel nanopore sequencing data characteristics.

Purpose of the Study:

  • To introduce NanoR, a novel quality control method specifically designed for nanopore sequencing data.
  • To contextualize NanoR within the landscape of existing quality control tools for nanopore sequencing.
  • To demonstrate the practical application of quality control pipelines using NanoR and PyPore.

Main Methods:

  • Development and application of the NanoR quality control method for nanopore sequencing data.
  • Comparative analysis of NanoR against other available quality control tools.
  • Implementation of two distinct quality control pipelines utilizing NanoR and PyPore.

Main Results:

  • NanoR provides a robust method for assessing nanopore sequencing data quality.
  • The developed pipelines offer practical solutions for quality control of nanopore sequencing outputs.
  • Evaluation of data quality is essential for maximizing the utility of nanopore sequencing in biological discovery.

Conclusions:

  • NanoR is a valuable addition to the toolkit for nanopore sequencing data analysis.
  • The presented quality control pipelines facilitate reliable interpretation of nanopore sequencing results.
  • Effective quality assessment is paramount for advancing biological discoveries using third-generation sequencing.