Structure of EDTA-treated satellite tobacco necrosis virus at pH 6.5

The crystal structure of EDTA-treated satellite tobacco necrosis virus (STNV) at pH 6.5 has been determined to 7.5 A resolution (1 A = 0.1 nm) with molecular replacement techniques, using the known structure of the protein subunit. The calcium ions at the 3-fold contacts are absent, whereas the calcium ions on the 5-fold axes still remain. The protein shell is slightly expanded. The expansion does not impose any large conformational changes on the subunits and the subunit contacts are to a large extent retained. The electron density map shows high levels of density in the RNA region. It is found close to the protein shell but well-separated from the protein. This density indicates a preferential ordering of the RNA in certain regions, but does not allow a detailed interpretation of the RNA conformation. A similar density in the RNA region is also found in a low resolution map of native STNV.