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High-Performance Liquid Chromatography: Introduction

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In HPLC, two phases play a critical role in the separation process:

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Related Experiment Video

Updated: Jan 8, 2026

HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis

HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis

Published on: November 11, 2022

A HPLC fingerprint study on Chaenomelis Fructus.

Lili Zhu¹, Lexia Fang¹, Zongjin Li¹

¹1College of Pharmacy, Anhui University of Chinese Medicine, Hefei, 230012 China.

|August 7, 2019

Summary

This study introduces a new HPLC method for identifying Chaenomelis Fructus quality. The chromatographic fingerprinting effectively distinguishes authentic samples from adulterated ones, ensuring traditional medicine integrity.

Keywords:

Chromatographic fingerprint HPLC Similarity

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Last Updated: Jan 8, 2026

HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis

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Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Published on: May 20, 2013

Area of Science:

Pharmacognosy
Analytical Chemistry
Traditional Chinese Medicine

Background:

Chaenomelis Fructus is a traditional Chinese medicine with quality control challenges.
Current quality standards rely on individual component analysis, lacking holistic control.
A novel approach is needed for rapid identification of chemical ingredients.

Purpose of the Study:

To develop and validate a High-Performance Liquid Chromatography (HPLC) method for Chaenomelis Fructus quality control.
To establish a chromatographic fingerprinting technique for rapid identification and authentication.
To differentiate authentic Chaenomelis Fructus from potential adulterants.

Main Methods:

Validated an HPLC method for precision, repeatability, and stability.
Analyzed 20 batches of Chaenomelis Fructus from geographical origins.
Utilized Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine software for analysis.

Main Results:

The HPLC method showed excellent precision, repeatability, and stability.
Chromatographic fingerprint analysis revealed high similarity among 19 samples, with one sample identified as potentially adulterated.
Principal component and hierarchical clustering analyses supported the identification of the adulterated sample.

Conclusions:

A characteristic and specific chromatographic fingerprint for Chaenomelis Fructus was established.
This fingerprint serves as a reliable reference for quality control.
The developed HPLC method provides a fast and effective tool for distinguishing authentic from adulterated Chaenomelis Fructus.