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[Effect of resveratrol on the expression of MIP-2 in P.e-LPS-induced mouse osteoblasts]
Ya-Qiong Yu1, Xiao-Lin Li, Li-Hong Qiu
1Department of Endodontics, School of Stomatology, China Medical University; Lab of Endodontics, Liaoning Institute of Dental Research; Liaoning Provincial Research Center of Translational Oral Medicine. Shenyang 110002, Liaoning Province, China.
Purpose:
To investigate the effect of lipopolysaccharides(LPS) extracted from Porphyromonas endodontalis(P.e) on the expression of macrophage inflammatory protein-2 (MIP-2) mRNA and protein levels in MC3T3-E1 cells and the influence of resveratrol on the expression of MIP-2 protein in P.e-LPS induced cells.
Methods:
MC3T3-E1 cells were treated with different concentrations of P.e-LPS(0-50 mg/L) and 20 mg/L P.e-LPS for different time (0-48 h). The expression of MIP-2 mRNA and protein was detected by real-time RT-PCR and enzyme linked immunosorbent assay (ELISA). MC3T3-E1 cells were pretreated with resveratrol for 1 h in the presence of 20 mg/L P.e-LPS for 24 h，which was detected by ELISA. Statistical analysis was performed using one-way ANOVA and Dunnett t test with SPSS 13.0 software package.
Results:
Treatment of MC3T3-El cells with different concentrations of P.e-LPS(0-50 mg/L) caused a significantly increase in MIP-2 mRNA and protein expression in dose-dependent manners.The expression of MIP-2 protein increased from (41.86±2.49) ng/L to (3126.74±158.30) ng/L, and the difference was significant(P<0.05). In the observation time (0-48 h), the impact of 20 mg/L P.e-LPS on induction of MIP-2 in MC3T3-El cells exhibited a time-dependent manner. At 48 h, the maximal induction of MIP-2 protein expression was (2102.55±123.27) ng/L(P<0.01). Incubation of cells with 10 μmol/L resveratrol for 1h significantly decreased the expression of MIP-2 protein from (1805.33±67.54) ng/L to（813.82±47.21) ng/L, and the difference was significant(P<0.05).
Conclusions:
The results suggest that P.e-LPS may mediate MIP-2 expression in MC3T3-E1 cells, and resveratrol has a significant inhibitory effect on this process.
Insights
Porphyromonas endodontalis lipopolysaccharides (P.e-LPS) significantly increase macrophage inflammatory protein-2 (MIP-2) in MC3T3-E1 cells. Resveratrol effectively inhibits this P.e-LPS-induced MIP-2 expression, suggesting a therapeutic potential.
Area of Science:
- Cell Biology
- Immunology
- Periodontology
Background:
- Porphyromonas endodontalis (P.e) is implicated in periodontal disease.
- Lipopolysaccharides (LPS) from P.e can trigger inflammatory responses.
- Macrophage inflammatory protein-2 (MIP-2) is a key chemokine in inflammation.
Purpose of the Study:
- To determine the effect of P.e-LPS on MIP-2 mRNA and protein expression in MC3T3-E1 cells.
- To investigate the inhibitory effect of resveratrol on P.e-LPS-induced MIP-2 expression.
Main Methods:
- MC3T3-E1 cells were treated with varying concentrations and durations of P.e-LPS.
- MIP-2 mRNA and protein levels were quantified using real-time RT-PCR and ELISA.
- Resveratrol's effect on P.e-LPS-induced MIP-2 was assessed via ELISA.
Main Results:
- P.e-LPS significantly upregulated MIP-2 mRNA and protein expression in a dose- and time-dependent manner.
- MIP-2 protein levels increased from baseline to 3126.74 ng/L with P.e-LPS treatment.
- Resveratrol (10 μmol/L) significantly reduced P.e-LPS-induced MIP-2 protein expression.
Conclusions:
- P.e-LPS plays a role in mediating MIP-2 expression in MC3T3-E1 cells.
- Resveratrol demonstrates a significant inhibitory effect on P.e-LPS-induced MIP-2 expression.
- These findings suggest a potential therapeutic role for resveratrol in P.e-LPS-related inflammatory conditions.
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