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Multilocus Sequence Typing (MLST) of Chlamydiales
Martina Jelocnik1, Adam Polkinghorne2, Yvonne Pannekoek3
1Genecology Research Centre, University of the Sunshine Coast, Sippy Downs, QLD, Australia.
Methods in Molecular Biology (Clifton, N.J.)
|August 7, 2019
Summary
Multilocus Sequence Typing (MLST) provides a universal molecular barcode for bacterial and eukaryotic organisms. This study details MLST methods for analyzing Chlamydiales bacteria, using a specific scheme for Chlamydia pecorum.
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Background:
- Multilocus Sequence Typing (MLST) is a widely adopted molecular method for bacterial and eukaryotic organism characterization.
- MLST relies on sequencing conserved housekeeping (HK) genes to generate allelic profiles, forming a unique sequence type (ST) or 'molecular barcode'.
Purpose of the Study:
- To describe the principles and molecular approaches for generating MLST data.
- To demonstrate the application of MLST for analyzing bacteria within the order Chlamydiales.
- To present a specific MLST scheme developed for Chlamydia pecorum.
Main Methods:
- Utilized DNA sequencing of conserved housekeeping genes.
- Assigned allelic numbers to gene sequences.
- Combined allelic profiles to define sequence types (STs).
Main Results:
- Established a Chlamydia pecorum-specific MLST scheme.
- Demonstrated the generation of MLST data for Chlamydiales analysis.
Conclusions:
- MLST is a powerful and versatile tool for fine-detailed molecular typing of diverse organisms.
- The described Chlamydia pecorum MLST scheme facilitates robust analysis within the Chlamydiales order.
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