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Neurosteroid pregnenolone sulfate antagonizes electrophysiological responses to GABA in neurons
M D Majewska1, J M Mienville, S Vicini
1FIDIA-Georgetown University for the Neurosciences, Georgetown University Medical School, Washington, DC 20007.
Neuroscience Letters
|August 1, 1988
Abstract:
Our earlier biochemical studies suggested that the neurosteroid pregnenolone sulfate (PS) may reduce gamma-aminobutyric acid (GABA) action at the Cl- channel associated with GABAA receptors. In the present electrophysiological study the interaction of PS with the GABAA receptor was tested, using whole-cell voltage-clamp recordings from isolated cerebral cortical neurons of neonatal rats. At micromolar concentrations PS reversibly inhibited GABA-induced current, behaving as an allosteric receptor antagonist.