Rigidity-Dependent Placental Cells Uptake of Silk-Based Microcapsules

Polymeric microcapsules have begun to attract significant interest in biomedical fields. As the interactions between cells and materials are influenced by both cell type and elasticity, silk-based microcapsules are synthesized with desirable mechanical features using layer-by-layer assembly and then the uptake of these microcapsules by BeWo b30 placental cells is investigated. Cellular uptake is enhanced with increasing of elastic modulus of the silk-based microcapsules. More importantly, the distinct microvilli of these cells behaves in a diverse manner when exposed to microcapsules with different mechanical features, including grabbing (rigidity) or random touching (soft) behavior; these factors affect the final uptake. Inspired by oocyte pickup, the grabbing behavior of the microvilli may provide valuable information with which to elucidate the specific characteristics of uptake between cells and man-made particles, particularly in the reproductive system.