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Updated: Jan 21, 2026

Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication
Published on: September 20, 2016
Importance of harmonised sample preparation for moisture and protein content determinations in official food control
Yannick Weesepoel1, Erika Silletti1, Martin Alewijn1
1Wageningen Food Safety Research, Part of Wageningen University and Research, P.O. Box 230, 6700 AE Wageningen, the Netherlands.
Abstract:
Commission Regulation (EC) 543/2008 limits moisture and protein contents in poultry meat. However, this regulation leaves room for interpretation regarding sample homogenisation, potentially affecting comparability of laboratory results. Therefore, a proficiency test and sample homogenisation study were organised amongst 19 European National Reference Laboratories (NRL). In the proficiency test, three different pre-homogenised chicken samples (fillets, drumsticks and carcasses) were analysed. Only one NRL produced unsatisfactory results. In the homogenisation study, NRLs were supplied with uniform fillet, drumstick and carcass materials. Homogenisation was performed according to the NRLs in-house methods. Five NRLs did not return satisfactory results. As these NRLs produced satisfactory results in the proficiency test, their increase in z-scores was related to their homogenisation practices. Overall, scattering of individual results was higher for drumsticks compared to fillets and carcasses. Homogenisation practices for poultry meat introduced significant differences in moisture and protein results and standardisation is therefore advisable.
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